Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1

Li, Zhiyong1,2,3; Fu, Yajuan2; Wang, Yuzhu2; Liang, Jiansheng1,2

2023-06-01

10.1111/nph.19049

NEW PHYTOLOGIST   Impact Factor & Quartile

0028-646X

1469-8137

["Brassinosteroids (BRs) are a group of plant-specific steroid hormones, which induces the rapid nuclear localization of the positive transcriptional factors BRASSINAZOLE RESISTANT1/2 (BZR1/2). However, the mechanisms underlying the regulation of nucleocytoplasmic shuttling of BZR1 remain to be fully elucidated.","In this study, we show that the scaffold protein Receptor for Activated C Kinase 1 (RACK1) from Arabidopsis is involved in BR signaling cascades through mediating the nuclear localization of BZR1, which is tightly retained in the cytosol by the conserved scaffold protein 14-33s.","RACK1 can interact with BZR1 and competitively decrease the 14-3-3 interaction with BZR1 in cytosol, which efficiently enhances the nuclear localization of BZR1. 14-3-3 also retains RACK1 in cytosol through their interaction. Conversely, BR treatment enhances the nuclear localization of BZR1 by disrupting the 14-3-3 interaction with RACK1 and BZR1.","Our study uncovers a new mechanism that integrates two kinds of conserved scaffold proteins (RACK1 and 14-3-3) coordinating BR signaling event."]

brassinosteroid BZR1 nucleocytoplasmic shuttling RACK1 scaffold protein

SCI

English

First ; Corresponding

Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes[2019KSYS006] ; National Natural Science Foundation of China[31801277]

Plant Sciences

Plant Sciences

WOS:001005129100001

WILEY

PLANT & ANIMAL SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory Guangdong Hi, Dept Biol, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China

Li, Zhiyong,Fu, Yajuan,Wang, Yuzhu,et al. Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1[J]. NEW PHYTOLOGIST,2023.

Li, Zhiyong,Fu, Yajuan,Wang, Yuzhu,&Liang, Jiansheng.(2023).Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1.NEW PHYTOLOGIST.

Li, Zhiyong,et al."Scaffold protein RACK1 regulates BR signaling by modulating the nuclear localization of BZR1".NEW PHYTOLOGIST (2023).