Identification and functional characterization of a bipartite nuclear localization signal in ANKRD11

Chen, Min1; Yang, Xue2; Liu, Haiyang2; Wan, Jun1,2,3

2023-08-30

10.1016/j.bbrc.2023.05.046

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Impact Factor & Quartile

0006-291X

1090-2104

ANKRD11 gene encodes for the large nuclear protein essential for multiple system development including the nervous system. However, the molecular basis for the proper nuclear localization of ANKRD11 has not yet been elucidated. In this study, we have identified a functional bipartite nuclear localization signal (bNLS) between residues 53 and 87 of ANKRD11. Using biochemical approaches, we discovered two major binding sites in this bipartite NLS for Importin a1. Through site-directed mutagenesis and func-tional analysis, we further found that this bipartite NLS is sufficient for nuclear import of overexpressing GFP in HeLa cells and necessary for nuclear localization of ANKRD11. Importantly, our study provides a possible pathogenic mechanism for certain clinical variants located within the bipartite nuclear locali-zation signal of ANKRD11. & COPY; 2023 Elsevier Inc. All rights reserved.

ANKRD11 Bipartite nuclear localization signal (bNLS) Nuclear import

SCI

English

Corresponding

Guangdong Innovative and Entre- preneurial Research Team Program[2021ZT09Y104] ; Shenzhen Science and Technology Basic Research Program[JCYJ20220818100215033]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:001016155200001

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Biomed Res Inst, Med Ctr, Shenzhen 518036, Peoples R China
2.Shenzhen Bay Lab, Greater Bay Biomed InnoCtr, Shenzhen 518107, Peoples R China
3.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China

Chen, Min,Yang, Xue,Liu, Haiyang,et al. Identification and functional characterization of a bipartite nuclear localization signal in ANKRD11[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2023,670.

Chen, Min,Yang, Xue,Liu, Haiyang,&Wan, Jun.(2023).Identification and functional characterization of a bipartite nuclear localization signal in ANKRD11.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,670.

Chen, Min,et al."Identification and functional characterization of a bipartite nuclear localization signal in ANKRD11".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 670(2023).