Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry

Qin, Yan1; He, Ying-Jie1,3; Zhao, Yun-Li1; Zhou, Zhong-Shun1; Wang, Zhao-Jie1; Zhu, Yan-Yan1; Luo, Xiao-Dong1,2

2023-06-01

10.1002/jssc.202200843

JOURNAL OF SEPARATION SCIENCE   Impact Factor & Quartile

1615-9306

1615-9314

Monoterpene indole alkaloids exhibit structural diversity in herbal resources and have been developed as promising drugs owing to their significant biological activities. Confidential identification and quantification of monoterpene indole alkaloids is the key to quality control of target plants in industrial production but has rarely been reported. In this study, quantitative performance of three data acquisition modes of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry including full scan, auto-MS2 and target-MS2, was evaluated and compared for specificity, sensitivity, linearity, precision, accuracy, and matrix effect using five monoterpene indole alkaloids (scholaricine, 19-epi-scholaricine, vallesamine, picrinine, and picralinal). Method validations indicated that target-MS2 mode showed predominant performance for simultaneous annotation and quantification of analytes, and was then applied to determine monoterpene indole alkaloids in Alstonia scholaris (leaves, barks) after extraction procedures optimization using Box-Behnken design of response surface methodology. The variations of A. scholaris monoterpene indole alkaloids in different plant parts, harvest periods, and post-handling processes, were subsequently investigated. The results indicated that target-MS2 mode could improve the quantitative capability of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for structure-complex monoterpene indole alkaloids in herbal matrices.Alstonia scholaris, monoterpene indole alkaloids, quadrupole time of flight mass spectrometry, qualitative and quantitative analysis, ultra-high-performance liquid chromatography

SCI

English

Others

High-level Talent Promotion and Training Project of Kunming[2022SCP003] ; Yunnan Characteristic Plant Screening and Ramp;D Service CXO Platform[2022YKZY001] ; Scientific and Technological Innovation Team of Yunnan Province[202105AE160006]

Chemistry

Chemistry, Analytical

WOS:001014416200001

WILEY-V C H VERLAG GMBH

CHEMISTRY

Web of Science

1.Yunnan Univ, Sch Chem Sci & Technol, Yunnan Characterist Plant Extract Lab, Key Lab Med Chem Nat Resource,Minist Educ & Yunnan, Kunming, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources Wes, Kunming, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, R China, Shenzhen, Peoples R China

Qin, Yan,He, Ying-Jie,Zhao, Yun-Li,et al. Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2023.

Qin, Yan.,He, Ying-Jie.,Zhao, Yun-Li.,Zhou, Zhong-Shun.,Wang, Zhao-Jie.,...&Luo, Xiao-Dong.(2023).Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry.JOURNAL OF SEPARATION SCIENCE.

Qin, Yan,et al."Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry".JOURNAL OF SEPARATION SCIENCE (2023).