Spliceosomal protein U2B" delays leaf senescence by enhancing splicing variant JAZ9 beta expression to attenuate jasmonate signaling in Arabidopsis

Yang, Qi1; Tan, Shuya1; Wang, Hou-Ling1; Wang, Ting1; Cao, Jie1; Liu, Hairong1; Sha, Yueqi1; Zhao, Yaning1; Xia, Xinli1; Guo, Hongwei2; Li, Zhonghai1

2023-08-01

10.1111/nph.19198

NEW PHYTOLOGIST   Impact Factor & Quartile

0028-646X

1469-8137

The regulatory framework of leaf senescence is gradually becoming clearer; however, the fine regulation of this process remains largely unknown. Here, genetic analysis revealed that U2 small nuclear ribonucleoprotein B (U2B 00), a component of the spliceosome, is a negative regulator of leaf senescence. Mutation of U2B" led to precocious leaf senescence, whereas overexpression of U2B" extended leaf longevity. Transcriptome analysis revealed that the jasmonic acid (JA) signaling pathway was activated in the u2b" mutant. U2B" enhances the generation of splicing variant JASMONATE ZIM-DOMAIN 9 beta (JAZ9 beta) with an intron retention in the Jas motif, which compromises its interaction with CORONATINE INSENSITIVE1 and thus enhances the stability of JAZ9 beta protein. Moreover, JAZ9 beta could interact with MYC2 and obstruct its activity, thereby attenuating JA signaling. Correspondingly, overexpression of JAZ9 beta rescued the early senescence phenotype of the u2b" mutant. Furthermore, JA treatment promoted expression of U2B" that was found to be a direct target of MYC2. Overexpression of MYC2 in the u2b" mutant resulted in a more pronounced premature senescence than that in wild-type plants. Collectively, our findings reveal that the spliceosomal protein U2B" fine-tunes leaf senescence by enhancing the expression of JAZ9 beta and thereby attenuating JA signaling.

jasmonic acid (JA) leaf senescence spliceosome transcription factor U2B"

SCI

English

Others

Nottingham Arabidopsis Stock Centre (NASC) - Beijing Municipal Natural Science Foundation[5232015] ; Open Research Fund of the National Center for Protein Sciences at Peking University in Beijing[KF-202304] ; National Natural Science Foundation of China["32170345","31970196"] ; Fundamental Research Funds for the Central Universities[GK122201280] ; National Key Research and Development Program of China[2019YFA0903904]

Plant Sciences

Plant Sciences

WOS:001052258100001

WILEY

PLANT & ANIMAL SCIENCE

Web of Science

1.Beijing Forestry Univ, Coll Biol Sci & Technol, State Key Lab Tree Genet & Breeding, Beijing 100083, Peoples R China
2.Southern Univ Sci & Technol SUSTech, Key Lab Mol Design Plant Cell Factory Guangdong Hi, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China

Yang, Qi,Tan, Shuya,Wang, Hou-Ling,et al. Spliceosomal protein U2B" delays leaf senescence by enhancing splicing variant JAZ9 beta expression to attenuate jasmonate signaling in Arabidopsis[J]. NEW PHYTOLOGIST,2023.

Yang, Qi.,Tan, Shuya.,Wang, Hou-Ling.,Wang, Ting.,Cao, Jie.,...&Li, Zhonghai.(2023).Spliceosomal protein U2B" delays leaf senescence by enhancing splicing variant JAZ9 beta expression to attenuate jasmonate signaling in Arabidopsis.NEW PHYTOLOGIST.

Yang, Qi,et al."Spliceosomal protein U2B" delays leaf senescence by enhancing splicing variant JAZ9 beta expression to attenuate jasmonate signaling in Arabidopsis".NEW PHYTOLOGIST (2023).