Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids

Feng, Yilin1,2; Zheng, Honghui1,2; Tang, Jiyuan1,2; Li, Xing3; Tian, Ruilin3; Ma, Shaohua1,2

2023-09-15

10.1016/j.xpro.2023.102346

STAR PROTOCOLS   Impact Factor & Quartile

2666-1667

In glioma modeling, existing organoid protocols lack the ability to replicate glioma cell invasion and interaction with normal brain tissue. Here, we present a protocol for generating in vitro brain disease models using human-induced pluripotent-or embryonic-stem-cell-derived cerebral organoids (COs). We describe steps for forming glioma organoids by co-culturing forebrain organoids with U-87 MG cells. We also detail vibratome sectioning of COs to prevent cell death and enhance contact between U-87 MG cells and cerebral tissues.

ESCI

English

Others

National Natural Science Foundation of China["82111530212","2021B1515020092"] ; Natural Science Foundation of Guangdong Province[SZBL2020090501 014] ; Shenzhen Bay Laboratory Fund[WDZC20 200821141349001] ; Shenzhen Science and Technology Innovation Commission["KCXFZ20200201101050887","RCYX20200714114736146","KCXFZ2020122117 3207022"] ; null[61971255]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:001058449000001

ELSEVIER

Web of Science

1.Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch SIGS, Shenzhen 518055, Peoples R China
2.Tsinghua Berkeley Shenzhen Inst TBSI, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol SUSTech, Sch Med, Shenzhen 518055, Peoples R China

Feng, Yilin,Zheng, Honghui,Tang, Jiyuan,et al. Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids[J]. STAR PROTOCOLS,2023,4(3).

Feng, Yilin,Zheng, Honghui,Tang, Jiyuan,Li, Xing,Tian, Ruilin,&Ma, Shaohua.(2023).Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids.STAR PROTOCOLS,4(3).

Feng, Yilin,et al."Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids".STAR PROTOCOLS 4.3(2023).