Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4+CD8+T cells expansion

Zhu，Min zheng1; Xu，Hao ming1; Liang，Yu jie2; Xu，Jing1; Yue，Ning ning3; Zhang，Yuan4; Tian，Cheng mei5; Yao，Jun6,7; Wang，Li sheng6,7; Nie，Yu qiang1; Li，De feng6,7

2023-12-01

10.1186/s12951-023-02065-0

Journal of Nanobiotechnology   Impact Factor & Quartile

1477-3155

Abstract: Plant-derived exosome-like nanoparticles (PDENs) have been paid great attention in the treatment of ulcerative colitis (UC). As a proof of concept, we isolated and identified Portulaca oleracea L-derived exosome-like nanoparticles (PELNs) from edible Portulaca oleracea L, which exhibited desirable nano-size (~ 160 nm) and a negative zeta potential value (-31.4 mV). Oral administration of PELNs effectively suppressed the expressions of pro-inflammatory cytokines (TNF-α, IL-6, IL-12, and IL-1β) and myeloperoxidase (MPO), increased levels of the anti-inflammatory cytokine (IL-10), and alleviated acute colitis in dextran sulfate sodium (DSS)-induced C57 mice and IL-10 mice. Notably, PELNs exhibited excellent stability and safety within the gastrointestinal tract and displayed specific targeting to inflamed sites in the colons of mice. Mechanistically, oral administration of PELNs played a crucial role in maintaining the diversity and balance of gut microbiota. Furthermore, PELNs treatment enhanced Lactobacillus reuteri growth and elevated indole derivative levels, which might activate the aryl-hydrocarbon receptor (AhR) in conventional CD4 T cells. This activation downregulated Zbtb7b expression, leading to the reprogramming of conventional CD4 T cells into double-positive CD4CD8T cells (DP CD4CD8 T cells). In conclusion, our findings highlighted the potential of orally administered PELNs as a novel, natural, and colon-targeted agent, offering a promising therapeutic approach for managing UC. Graphic abstract: Schematic illustration of therapeutic effects of oral Portulaca oleracea L -derived natural exosome-like nanoparticles (PELNs) on UC. PELNs treatment enhanced Lactobacillus reuteri growth and elevated indole derivative levels, which activate the aryl-hydrocarbon receptor (AhR) in conventional CD4 T cells leading to downregulate the expression of Zbtb7b, reprogram of conventional CD4 T cells into double-positive CD4CD8T cells (DP CD4CD8 T cells), and decrease the levels of pro-inflammatory cytokines. [Figure not available: see fulltext.]

Double-positive CD4+CD8+T Lactobacillus reuteri Portulaca oleracea L-derived exosome-like nanoparticles Ulcerative colitis

SCI

English

Corresponding

Basic and Applied Basic Research Foundation of Guangdong Province[2021A1515010985];Basic and Applied Basic Research Foundation of Guangdong Province[2023A1515011936];

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Nanoscience & Nanotechnology

WOS:001059590900001

BMC

BIOLOGY & BIOCHEMISTRY

2-s2.0-85169229562

Scopus

1.Department of Gastroenterology and Hepatology,the Second Affiliated Hospital,School of Medicine,South China University of Technology,Guangzhou,Guangdong,510006,China
2.School of Rehabilitation Medicine,Jining Medical University,Jining,Shandong,272029,China
3.Department of Gastroenterology,Shenzhen People’s Hospital,the Second Clinical Medical College,Jinan University,Shenzhen,Guangdong,518020,China
4.Department of Medical Administration,Huizhou Institute of Occupational Diseases Control and Prevention,Huizhou,Guangdong,516000,China
5.Department of Emergency,Shenzhen People’s Hospital,the Second Clinical Medical College,the First Affiliated Hospital,Jinan University,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
6.Department of Gastroenterology,Shenzhen People’s Hospital,the Second Clinical Medical College,the First Affiliated Hospital,Jinan University,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
7.Shenzhen Clinical Research Centre for Geriatrics,Shenzhen People’s Hospital,Shenzhen,Guangdong,518020,China

Zhu，Min zheng,Xu，Hao ming,Liang，Yu jie,et al. Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4+CD8+T cells expansion[J]. Journal of Nanobiotechnology,2023,21(1).

Zhu，Min zheng.,Xu，Hao ming.,Liang，Yu jie.,Xu，Jing.,Yue，Ning ning.,...&Li，De feng.(2023).Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4+CD8+T cells expansion.Journal of Nanobiotechnology,21(1).

Zhu，Min zheng,et al."Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4+CD8+T cells expansion".Journal of Nanobiotechnology 21.1(2023).