YY1-regulated lncRNA SOCS2-AS1 suppresses HCC cell stemness and progression via miR-454-3p/CPEB1

Zhong，Feng1; Wang，Yuanxi2

2023-10-30

10.1016/j.bbrc.2023.08.056

Biochemical and Biophysical Research Communications   Impact Factor & Quartile

0006-291X

1090-2104

Background: Cancer stem cells are one fundamental reason for the high recurrence rate of hepatocellular carcinoma (HCC) and its resistance to treatment. This study explored the mechanism by which SOCS2-AS1 affects HCC cell stemness. Methods: Stem cells of HCC cell lines Huh7 and SNU-398 were sorted as NANOG-positive by flow cytometry. Stem cell sphere formation ability was detected. Stem cell viability, migration, invasion, and apoptosis were assessed by colony formation assays, Transwell assays, wound-healing assays, and TUNEL assays, respectively. The binding sites for SOCS2-AS1, miR-454-3p, miR-454-3p, and CPEB1 mRNA were assessed by dual-luciferase reporter assays. Quantitative real-time PCR (qPCR) and Western blot studies were performed to evaluate gene expression levels. ChIP and EMSA assays were conducted to confirm that YY1 binds with the SOCS2-AS1 promoter. A subcutaneous xenograft model was used to verify results in vivo. Tumor tissues were analyzed by H&E and TUNEL staining. Results: SOCS2-AS1 was expressed at low levels in NANOG HCC stem cells, and HCC patients with a high level of SOCS2-AS1 expression had a higher survival rate. SOCS2-AS1 inhibited HCC cell stemness, migration, and invasion, and increased the cisplatin sensitivity of HCC cells by regulating miR-454-3p/CPEB1. YY1 was confirmed as a transcription factor of SOCS2-AS1, and served to inhibit SOCS2-AS1 transcription. YY1 knockdown suppressed HCC stemness via SOCS2-AS1. The role of SOCS2-AS1 was confirmed in a subcutaneous xenograft model, and SOCS2-AS1 overexpression enhanced the inhibitory effect of cisplatin on HCC in vivo. Conclusions: YY1-regulated lncRNA SOCS2-AS1 suppresses HCC cell stemness and progression via miR-454-3p/CPEB1.

CPEB1 Hepatocellular carcinoma miR-454-3p SOCS2-AS1 Stemness

SCI

English

First

WOS:001071147700001

BIOLOGY & BIOCHEMISTRY

2-s2.0-85169790509

Scopus

1.Department of General Surgery,Hospital of Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
2.Vascular and Endovascular Surgery,Shenzhen Samii Medical Center,Shenzhen,Guangdong,518118,China

Zhong，Feng,Wang，Yuanxi. YY1-regulated lncRNA SOCS2-AS1 suppresses HCC cell stemness and progression via miR-454-3p/CPEB1[J]. Biochemical and Biophysical Research Communications,2023,679:98-109.

Zhong，Feng,&Wang，Yuanxi.(2023).YY1-regulated lncRNA SOCS2-AS1 suppresses HCC cell stemness and progression via miR-454-3p/CPEB1.Biochemical and Biophysical Research Communications,679,98-109.

Zhong，Feng,et al."YY1-regulated lncRNA SOCS2-AS1 suppresses HCC cell stemness and progression via miR-454-3p/CPEB1".Biochemical and Biophysical Research Communications 679(2023):98-109.