A universal stimuli-responsive biosensor for disease biomarkers through their cysteine residues: A proof of concept on carcinoembryonic antigen (CEA) biomarker

Gholami，Mahnaz D.1; Nihal，Serena1; Liu，Qian2; Sarfo，Daniel1,3; Sonar，Prashant1,3; Izake，Emad L.1,3

2023-10-15

10.1016/j.snb.2023.134208

Sensors and Actuators B: Chemical   Impact Factor & Quartile

0925-4005

Carcinoembryonic antigen (CEA) is the gold standard biomarker for the clinical diagnosis of cancers diseases. Accurate, fast, and sensitive screening of CEA plays an important role in cancer disease detection and prognosis. This work reports the development of a new stimuli-responsive biosensor that determines the CEA blood plasma concentration within 10 min. Tert-butyl carbonate Naphthalene-flanked diketopyrroloprrole (mono-TBC-DPPN) fluorescent agent was synthesised, characterised and immobilised gold nanoparticles to develop the stimuli-responsive sensor. The target protein was purified from spiked plasma (from human blood), using an antibody-coated gold substrate. The purified CEA was then chemically modified to provide thiol groups (SH) that interact with the mono-TBC-DPPN on the surface of the stimuli-responsive biosensor to spontaneously displace it, thus leading to its fluorescence emission at 532 nm to a turn on. The new method and sensor was implemented to quantify CEA in spiked blood plasma down to 10 pg.mL within 45 min (35 min for the protein purification and modification and 10 min for its quantification using the sensor)

Cancer diagnosis CEA protein Mono-TBC-DPPN Paper based sensor Stimuli responsive biosensor

English

Others

CHEMISTRY

2-s2.0-85164214539

Scopus

1.School of Chemistry and Physics,Faculty of Science,Queensland University of Technology (QUT),Brisbane,2 George Street,4000,Australia
2.Department of Electronic and Electrical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.Centre for Materials Science,Queensland University of Technology (QUT),Brisbane,4000,Australia

Gholami，Mahnaz D.,Nihal，Serena,Liu，Qian,et al. A universal stimuli-responsive biosensor for disease biomarkers through their cysteine residues: A proof of concept on carcinoembryonic antigen (CEA) biomarker[J]. Sensors and Actuators B: Chemical,2023,393.

Gholami，Mahnaz D.,Nihal，Serena,Liu，Qian,Sarfo，Daniel,Sonar，Prashant,&Izake，Emad L..(2023).A universal stimuli-responsive biosensor for disease biomarkers through their cysteine residues: A proof of concept on carcinoembryonic antigen (CEA) biomarker.Sensors and Actuators B: Chemical,393.

Gholami，Mahnaz D.,et al."A universal stimuli-responsive biosensor for disease biomarkers through their cysteine residues: A proof of concept on carcinoembryonic antigen (CEA) biomarker".Sensors and Actuators B: Chemical 393(2023).