RNA helicase DHX33 regulates HMGB family genes in human cancer cells

Wang，Xingshun1; Chen，Shiyun2,3; Wen，Fuyu2; Zeng，Yong1; Zhang，Yandong2

2023-10-01

10.1016/j.cellsig.2023.110832

Cellular Signalling   Impact Factor & Quartile

0898-6568

1873-3913

RNA helicase DHX33 has been shown to be aberrantly expressed in various human cancers, however, its role in tumorigenesis remains incompletely understood. In this report, we uncovered that a family of DNA architecture proteins, HMGBs, can be regulated by DHX33 in cancer cells but not in normal cells. Specifically, DHX33 knockdown caused the downregulation of HMGBs at the levels of both gene transcription and protein expression. Notably, in RAS driven lung tumorigenesis, nuclear HMGBs proteins can be induced via DHX33. When DHX33 was knocked out, HMGBs overexpression was debilitated. Mechanistically, DHX33 was found to bind to the promoters of HMGB family genes and regulated their transcription through demethylation on gene promoters. Our study reveals a novel mechanism for DHX33 to promote tumorigenesis and highlights its therapeutic value in human cancers.

cancer DHX33 Epigenetic HMGB RNA helicase

English

Others

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85167597763

Scopus

1.The Sixth Affiliated Hospital of Kunming Medical University,Yuxi,Yunnan,653199,China
2.Shenzhen KeYe Life Technologies,Co.,Ltd,Shenzhen,Guangdong,518122,China
3.Southern University of Science and Technology,Shenzhen,China

Wang，Xingshun,Chen，Shiyun,Wen，Fuyu,et al. RNA helicase DHX33 regulates HMGB family genes in human cancer cells[J]. Cellular Signalling,2023,110.

Wang，Xingshun,Chen，Shiyun,Wen，Fuyu,Zeng，Yong,&Zhang，Yandong.(2023).RNA helicase DHX33 regulates HMGB family genes in human cancer cells.Cellular Signalling,110.

Wang，Xingshun,et al."RNA helicase DHX33 regulates HMGB family genes in human cancer cells".Cellular Signalling 110(2023).