Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus

Song，Xiehai1; Guo，Pengru2,3; Xia，Keke3; Wang，Meiling1; Liu，Yongqi1; Chen，Lichuan3; Zhang，Jinhui1; Xu，Mengyuan2,3; Liu，Naixu1; Yue，Zhiliang1; Xu，Xun3; Gu，Ying3; Li，Gang4; Liu，Min5; Fang，Liang6; Deng，Xing Wang1; Li，Bosheng1

2023-09-19

10.1073/pnas.2310163120

Proceedings of the National Academy of Sciences of the United States of America   Impact Factor & Quartile

1091-6490

Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation in crop engineering. Pluripotent callus cells develop into fertile shoots through shoot regeneration. The molecular basis of the shoot regeneration process in crop callus remains largely elusive. This study pioneers the exploration of the spatial transcriptome of tomato callus during shoot regeneration. The findings reveal the presence of highly heterogeneous cell populations within the callus, including epidermis, vascular tissue, shoot primordia, inner callus, and outgrowth shoots. By characterizing the spatially resolved molecular features of shoot primordia and surrounding cells, specific factors essential for shoot primordia formation are identified. Notably, chlorenchyma cells, enriched in photosynthesis-related processes, play a crucial role in promoting shoot primordia formation and subsequent shoot regeneration. Light is shown to promote shoot regeneration by inducing chlorenchyma cell development and coordinating sugar signaling. These findings significantly advance our understanding of the cellular and molecular aspects of shoot regeneration in tomato callus and demonstrate the immense potential of spatial transcriptomics in plant biology.

callus cellular heterogeneity chlorenchyma cells shoot regeneration spatial transcriptome

English

NI Journal Papers

Others

BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING

2-s2.0-85171232850

Scopus

1.Peking University Institute of Advanced Agricultural Sciences,Shandong Laboratory of Advanced Agriculture Sciences in Weifang,Weifang,China
2.Beijing Genomics Institute Research,102601,China
3.Beijing Genomics Institute Research,Shenzhen,518083,China
4.State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai'an,271018,China
5.
6.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518005,China

Song，Xiehai,Guo，Pengru,Xia，Keke,et al. Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus[J]. Proceedings of the National Academy of Sciences of the United States of America,2023,120(38).

Song，Xiehai.,Guo，Pengru.,Xia，Keke.,Wang，Meiling.,Liu，Yongqi.,...&Li，Bosheng.(2023).Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus.Proceedings of the National Academy of Sciences of the United States of America,120(38).

Song，Xiehai,et al."Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus".Proceedings of the National Academy of Sciences of the United States of America 120.38(2023).