Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq
|Joint first author||Li，Hui; Liu，Hongyi; Liu，Yifei|
T cell activation is a key event in adaptive immunity. However, the dynamics and influencing factors of T cell activation remain unclear. Here, we analyzed CD4 T cells that were stimulated with anti-CD3/CD28 under several conditions to explore the factors affecting T cell activation. We found a stimulated T subset (HSP T) highly expressing heat shock proteins, which was derived from stimulated naive T. We identified and characterized inert T, a stimulated T cell subset in transitional state from resting T to activated T. Interestingly, resting CXCR4 T responded to stimulation more efficiently than resting CXCR4 T. Furthermore, stimulation of CD4 T in the presence of CD8 T resulted in more effector T and more homogeneous expressions of CD25, supporting that presence of CD8 T reduces the extreme response of T cells, which can be explained by regulation of CD4 T activation through CD8 T-initiated cytokine signaling and FAS/FASLG signaling.
First ; 共同第一 ; Corresponding
National Key Research and Development Program of China[2021YFA0909300] ; National Key Research and Development Program of China[2021YFF1200900] ; Basic and Applied Basic Research Foundation of Guangdong Province[2023A1515011908] ; National Natural Science Foundation of China
Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||School of Life Sciences|
1.School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
2.CAS Key Laboratory of Computational Biology,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,200031,China
|First Author Affilication||School of Life Sciences|
|Corresponding Author Affilication||School of Life Sciences|
|First Author's First Affilication||School of Life Sciences|
Li，Hui,Liu，Hongyi,Liu，Yifei,et al. Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq[J]. iScience,2023,26(9).
Li，Hui.,Liu，Hongyi.,Liu，Yifei.,Wang，Xuefei.,Yu，Shiya.,...&Jin，Wenfei.(2023).Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq.iScience,26(9).
Li，Hui,et al."Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq".iScience 26.9(2023).
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