Amino Acid Profiling as A Screening and Prognostic Biomarker in Active Tuberculosis Patients
Background: Tuberculosis (TB) is one of the world's most deadly chronic infectious diseases; early diagnosis contributes to reducing disease transmission among populations. However, discovering novel diagnostic and prognostic biomarkers is still an important topic in the field of TB. Amino acid is the basic unit of protein composition, and its structure and physicochemical characteristics are more stable. Therefore, it is a potential target for TB diagnosis and the prediction of TB development. Methods: In this study, the blood of healthy people (HC), TB patients (TB), cured TB (RxTB), and other non-TB pneumonia patients (PN) were collected to detect the levels of amino acids in whole blood and plasma using ultra-high performance liquid chromatography coupled with mass spectrometry. Results: We detected that the amino acid levels correlated with participants status (TB, HC, RxTB, or PN) and the degree of lung damage. The results showed that phenylalanine had a good effect on the screening of TB (AUC = 0.924). We then built a TB prediction model. The model, which was based on the ratio of plasma amino acid content to whole blood amino acid content, showed good performance for the screening of TB, with 84% (95% CI = 60–97) sensitivity and 97% (95% CI = 83–100) specificity. The result the of correlation between the HRCT score and amino acid level indicated that the glutamine content of plasma was significantly inversely associated with disease severity. Additionally, ornithine levels in the plasma of RxTB group reduced and four amino acids of which the ratio in plasma to whole blood showed significantly changed. Conclusions: Taken together, amino acid profiling can be used for TB screening, and a multiparameter profiling model is better. The profiling can also reflect the severity of lung damage. Moreover, the amino acid profile is useful for reflecting the efficacy of TB treatment.
National Natural Science Founda-tion of China ; National Key Research and Devel-opment Plan[2021YFA1300902] ; Guangdong Science Fund for Distinguished Young Scholars ; Shenzhen Scientific and Technological Foundation["KCXFZ20211020163545004","KCXFZ202002011007083","JCYJ201802 28162453330","RCJC20221008092726022"]
|WOS Research Area|
Medical Laboratory Technology
Medical Laboratory Technology
|WOS Accession No|
|ESI Research Field|
Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||The Third People's Hospital of Shenzhen|
1.National Clinical Research Center for Infectious Diseases,Guangdong Provincial Clinical Research Center for Tuberculosis,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,518112,China
2.Department of Mass Spectrometry,BGI-Shenzhen,518083,Shenzhen,China
3.School of Basic Medical Sciences,Guangdong Medical University,Dongguan,523808,China
4.Department of Pathogen Biology,School of Medicine,Shenzhen University,Shenzhen,518037,China
|First Author Affilication||The Third People's Hospital of Shenzhen|
|First Author's First Affilication||The Third People's Hospital of Shenzhen|
Sun，Yunmei,Liao，Yunli,Xiong，Nating,et al. Amino Acid Profiling as A Screening and Prognostic Biomarker in Active Tuberculosis Patients[J]. Clinica Chimica Acta,2023,548.
Sun，Yunmei.,Liao，Yunli.,Xiong，Nating.,He，Xing.,Zhang，Huihua.,...&Zhang，Guoliang.(2023).Amino Acid Profiling as A Screening and Prognostic Biomarker in Active Tuberculosis Patients.Clinica Chimica Acta,548.
Sun，Yunmei,et al."Amino Acid Profiling as A Screening and Prognostic Biomarker in Active Tuberculosis Patients".Clinica Chimica Acta 548(2023).
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