Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish
Tissue-resident macrophages (TRMs) and dendritic cells (DCs) are highly heterogeneous and essential for immunity, tissue regeneration, and homeostasis maintenance. Here, we comprehensively profile the heterogeneity of TRMs and DCs across adult zebrafish organs via single-cell RNA sequencing. We identify two macrophage subsets: pro-inflammatory macrophages with potent phagocytosis signatures and pro-remodeling macrophages with tissue regeneration signatures in barrier tissues, liver, and heart. In parallel, one conventional dendritic cell (cDC) population with prominent antigen presentation capacity and plasmacytoid dendritic cells (pDCs) featured by anti-virus properties are also observed in these organs. Remarkably, in addition to a single macrophage/microglia population with potent phagocytosis capacity, a pDC population and two distinct cDC populations are identified in the brain. Finally, we generate specific reporter lines for in vivo tracking of macrophage and DC subsets. Our study depicts the landscape of TRMs and DCs and creates valuable tools for in-depth study of these cells in zebrafish.
National Key Research and Development Program of China[2018YFA0800200];Innovation and Technology Commission[ITCPD/17-9];
|WOS Research Area|
|WOS Accession No|
Cited Times [WOS]:2
|Document Type||Journal Article|
|Department||Department of Immunology and Microbiology|
1.Division of Life Science,State Key Laboratory of Molecular Neuroscience,the Hong Kong University of Science and Technology,Hong Kong,Clear Water Bay,Hong Kong
2.Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,518055,China
3.Department of Immunology and Microbiology,School of Life Science,Southern University of Science and Technology,Shenzhen,518055,China
|Corresponding Author Affilication||Department of Immunology and Microbiology|
Zhou，Qiuxia,Zhao，Changlong,Yang，Zhiyong,et al. Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish[J]. Cell Reports,2023,42(7).
Zhou，Qiuxia.,Zhao，Changlong.,Yang，Zhiyong.,Qu，Rui.,Li，Yunbo.,...&Wen，Zilong.(2023).Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish.Cell Reports,42(7).
Zhou，Qiuxia,et al."Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish".Cell Reports 42.7(2023).
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