Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish

Zhou，Qiuxia1; Zhao，Changlong1; Yang，Zhiyong1; Qu，Rui1; Li，Yunbo1; Fan，Yining1; Tang，Jinlin1; Xie，Ting1; Wen，Zilong1,2,3

2023-07-25

10.1016/j.celrep.2023.112793

Cell Reports   Impact Factor & Quartile

2211-1247

2211-1247

Tissue-resident macrophages (TRMs) and dendritic cells (DCs) are highly heterogeneous and essential for immunity, tissue regeneration, and homeostasis maintenance. Here, we comprehensively profile the heterogeneity of TRMs and DCs across adult zebrafish organs via single-cell RNA sequencing. We identify two macrophage subsets: pro-inflammatory macrophages with potent phagocytosis signatures and pro-remodeling macrophages with tissue regeneration signatures in barrier tissues, liver, and heart. In parallel, one conventional dendritic cell (cDC) population with prominent antigen presentation capacity and plasmacytoid dendritic cells (pDCs) featured by anti-virus properties are also observed in these organs. Remarkably, in addition to a single macrophage/microglia population with potent phagocytosis capacity, a pDC population and two distinct cDC populations are identified in the brain. Finally, we generate specific reporter lines for in vivo tracking of macrophage and DC subsets. Our study depicts the landscape of TRMs and DCs and creates valuable tools for in-depth study of these cells in zebrafish.

CP: Immunology dendritic cells macrophages mpeg1.1 scRNA-seq zebrafish

SCI

English

Corresponding

National Key Research and Development Program of China[2018YFA0800200];Innovation and Technology Commission[ITCPD/17-9];

Cell Biology

Cell Biology

WOS:001040315800001

CELL PRESS

2-s2.0-85165006378

Scopus

1.Division of Life Science,State Key Laboratory of Molecular Neuroscience,the Hong Kong University of Science and Technology,Hong Kong,Clear Water Bay,Hong Kong
2.Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,518055,China
3.Department of Immunology and Microbiology,School of Life Science,Southern University of Science and Technology,Shenzhen,518055,China

Zhou，Qiuxia,Zhao，Changlong,Yang，Zhiyong,et al. Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish[J]. Cell Reports,2023,42(7).

Zhou，Qiuxia.,Zhao，Changlong.,Yang，Zhiyong.,Qu，Rui.,Li，Yunbo.,...&Wen，Zilong.(2023).Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish.Cell Reports,42(7).

Zhou，Qiuxia,et al."Cross-organ single-cell transcriptome profiling reveals macrophage and dendritic cell heterogeneity in zebrafish".Cell Reports 42.7(2023).