Optical coherence tomography-based design for a real-time motion corrected scanning microscope

Tucker，Stephen S.1; Giblin，John T.1; Kiliç，Kivilcim1; Chen，Anderson1; Tang，Jianbo2; Boas，David A.1

2023-07-15

10.1364/OL.490087

Optics Letters   Impact Factor & Quartile

0146-9592

1539-4794

While two-photon fluorescence microscopy is a powerful platform for the study of functional dynamics in living cells and tissues, the bulk motion inherent to these applications causes distortions. We have designed a motion tracking module based on spectral domain optical coherence tomography which compliments a laser scanning two-photon microscope with real-time corrective feedback. The module can be added to fluorescent imaging microscopes using a single dichroic and without additional contrast agents. We demonstrate that the system can track lateral displacements as large as 10 µm at 5 Hz with latency under 14 ms and propose a scheme to extend the system to 3D correction with the addition of a remote focusing module. We also propose several ways to improve the module’s performance by reducing the feedback latency. We anticipate that this design can be adapted to other imaging modalities, enabling the study of samples subject to motion artifacts at higher resolution.

SCI

English

Others

Division of Biological Infrastructure[1934288];

Optics

Optics

WOS:001043404700006

Optica Publishing Group

PHYSICS

2-s2.0-85164753987

Scopus

1.Department of Biomedical Engineering,Boston University,Boston,44 Cummington Mall,02215,United States
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Tucker，Stephen S.,Giblin，John T.,Kiliç，Kivilcim,et al. Optical coherence tomography-based design for a real-time motion corrected scanning microscope[J]. Optics Letters,2023,48(14):3805-3808.

Tucker，Stephen S.,Giblin，John T.,Kiliç，Kivilcim,Chen，Anderson,Tang，Jianbo,&Boas，David A..(2023).Optical coherence tomography-based design for a real-time motion corrected scanning microscope.Optics Letters,48(14),3805-3808.

Tucker，Stephen S.,et al."Optical coherence tomography-based design for a real-time motion corrected scanning microscope".Optics Letters 48.14(2023):3805-3808.