Early and Sensitive Detection of Pathogens for Public Health and Biosafety: An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor
|Corresponding Author||Shi，Xin; Li，Xuejin; Zhang，Han|
Infectious diseases severely threaten public health and global biosafety. In addition to transmission through the air, pathogenic microorganisms have also been detected in environmental liquid samples, such as sewage water. Conventional biochemical detection methodologies are time-consuming and cost-ineffective, and their detection limits hinder early diagnosis. In the present study, ultrafine plasmonic fiber probes with a diameter of 125 μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface, causing a sharp reduction in the surface plasmon resonance (SPR) wavelength. The proposed fiber probe is virus-specific with the limit of detection of ~2,300 copies/ml, and genomic copy numbers can be reflected as shifts in wavelengths. A total of 21 sewage water samples have been examined, and the data obtained are consistent with those of quantitative polymerase chain reaction (qPCR). In addition, the Omicron variant and its mutation sites have been fast detected using S gene-specific Cas12a. This study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.
Doctoral Program Foundation of Institutions of Higher Education of China;Guangxi Higher Institutions Scientific Research Project[827-000636];Science, Technology and Innovation Commission of Shenzhen Municipality[JCJY20200109105608771];Ras Al Khaimah Center for Advanced Materials[RCAMS/KKU/0010/21];National Natural Science Foundation of China[U1813207];Major State Basic Research Development Program of China[YS2022YFB3200011];
Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||The Third People's Hospital of Shenzhen|
1.College of Physics and Optoelectronic Engineering,Institute of Translational Medicine,Department of Otolaryngology,Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University,Health Science Center,Shenzhen University,Shenzhen,518060,China
2.Shenzhen Key Laboratory of Sensor Technology,Shenzhen,518060,China
3.The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital,Qingyuan,511518,China
4.Shenzhen Key Laboratory of Pathogen and Immunity,National Clinical Research Center for Infectious Disease,State Key Discipline of Infectious Disease,Shenzhen Third People's Hospital,Second Hospital Affiliated to Southern University of Science and Technology,Shenzhen,518112,China
5.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
6.School of Physics and Optoelectronic Engineering,Shandong University of Technology,Zibo,255049,China
7.Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah,21589,Saudi Arabia
8.Research Center for Advanced Materials Science (RCAMS),King Khalid University,Abha,P.O. Box9004,61413,Saudi Arabia
9.Department of Chemistry,College of Science,King Khalid University,Abha,P.O. Box9004,61413,Saudi Arabia
10.Health Sciences Institute,China Medical University,Shenyang,110000,China
11.The Chinese University of HongKong,Shenzhen,518060,China
Li，Tianzhong,Chen，Yuzhi,Chen，Zhi,et al. Early and Sensitive Detection of Pathogens for Public Health and Biosafety: An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor[J]. Research,2023,6.
Li，Tianzhong.,Chen，Yuzhi.,Chen，Zhi.,Hao，Yuan.,Liang，Minyi.,...&Zhang，Han.(2023).Early and Sensitive Detection of Pathogens for Public Health and Biosafety: An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor.Research,6.
Li，Tianzhong,et al."Early and Sensitive Detection of Pathogens for Public Health and Biosafety: An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor".Research 6(2023).
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