Optical Control of Protein Functions via Genetically Encoded Photocaged Aspartic Acids

Zhang，Xianrui1; Huang，Haoran2; Liu，Yuan1; Wu，Zhigang2; Wang，Fengzhang1; Fan，Xinyuan1; Chen，Peng R.1,3; Wang，Jie2

2023

10.1021/jacs.3c03701

Journal of the American Chemical Society   Impact Factor & Quartile

0002-7863

1520-5126

Site-specific protein decaging by light has become an effective approach for in situ manipulation of protein activities in a gain-of-function fashion. Although successful decaging of amino acid side chains of Lys, Tyr, Cys, and Glu has been demonstrated, this strategy has not been extended to aspartic acid (Asp), an essential amino acid residue with a range of protein functions and protein-protein interactions. We herein reported a genetically encoded photocaged Asp and applied it to the photocontrolled manipulation of a panel of proteins including firefly luciferase, kinases (e.g., BRAF), and GTPase (e.g., KRAS) as well as mimicking the in situ phosphorylation event on kinases. As a new member of the increasingly expanded amino acid-decaging toolbox, photocaged Asp may find broad applications for gain-of-function study of diverse proteins as well as biological processes in living cells.

SCI

English

NI Journal Papers

Corresponding

National Key Research and Development Program of China["2019YFA0904201","2022YFA1304700","2021YFA1302603","2021YFA0910800"] ; National Natural Science Foundation of China["22222701","22137001","22077004","21937001","91957101","22077059","22277048","92253304","92253301"] ; Beijing Natural Science Foundation[Z200010] ; Young Elite Scientists Sponsorship Program from China Association for Science and Technology[YESS20200010] ; Shenzhen Innovation of Science and Technology Commission Grant["JCYJ20210324104210028","RCYX20210609103118010"]

Chemistry

Chemistry, Multidisciplinary

WOS:001063226100001

AMER CHEMICAL SOC

2-s2.0-85169847179

Scopus

1.Beijing National Laboratory for Molecular Sciences,Synthetic and Functional Biomolecules Center,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking University,Beijing,100871,China
2.Department of Chemistry,Research Center for Chemical Biology and Omics Analysis,College of Science,Guangdong Provincial Key Laboratory of Catalysis,Southern University of Science and Technology,Shenzhen,518055,China
3.Peking-Tsinghua Center for Life Sciences,Peking University,Beijing,100871,China

Zhang，Xianrui,Huang，Haoran,Liu，Yuan,et al. Optical Control of Protein Functions via Genetically Encoded Photocaged Aspartic Acids[J]. Journal of the American Chemical Society,2023,145(35).

Zhang，Xianrui.,Huang，Haoran.,Liu，Yuan.,Wu，Zhigang.,Wang，Fengzhang.,...&Wang，Jie.(2023).Optical Control of Protein Functions via Genetically Encoded Photocaged Aspartic Acids.Journal of the American Chemical Society,145(35).

Zhang，Xianrui,et al."Optical Control of Protein Functions via Genetically Encoded Photocaged Aspartic Acids".Journal of the American Chemical Society 145.35(2023).