| Title | Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system |
| Author | |
| Corresponding Author | Chen，Jian; Lai，Jinsheng |
| Publication Years | 2023-11-01
|
| DOI | |
| Source Title | |
| ISSN | 1467-7644
|
| EISSN | 1467-7652
|
| Abstract | The CRISPR-Cas systems have been widely used as genome editing tools, with type II and V systems typically introducing small indels, and type I system mediating long-range deletions. However, the precision of type I systems for large fragment deletion is still remained to be optimized. Here, we developed a compact Cascade-Cas3 Dvu I-C system with Cas11c for plant genome editing. The Dvu I-C system was efficient to introduce controllable large fragment deletion up to at least 20 kb using paired crRNAs. The paired-crRNAs design also improved the controllability of deletions for the type I-E system. Dvu I-C system was sensitive to spacer length and mismatch, which was benefit for target specificity. In addition, we showed that the Dvu I-C system was efficient for generating stable transgenic lines in maize and rice with the editing efficiency up to 86.67%. Overall, Dvu I-C system we developed here is powerful for achieving controllable large fragment deletions. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Others
|
| Funding Project | Hainan Yazhou Bay Seed Lab[B21HJ0509]
; Sanya Yazhou Bay Science and Technology City Administration[
|
| WOS Research Area | Biotechnology & Applied Microbiology
; Plant Sciences
|
| WOS Subject | Biotechnology & Applied Microbiology
; Plant Sciences
|
| WOS Accession No | WOS:001063754500001
|
| Publisher | |
| ESI Research Field | PLANT & ANIMAL SCIENCE
|
| Scopus EID | 2-s2.0-85168894315
|
| Data Source | Scopus
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/560114 |
| Department | Institute of Advanced Biotechnology 生命科学学院 |
| Affiliation | 1.State Key Laboratory of Maize Bio-breeding,National Maize Improvement Center,Department of Plant Genetics and Breeding,China Agricultural University,Beijing,China 2.Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China 3.University of Chinese Academy of Sciences,Beijing,China 4.College of Biological Sciences,China Agricultural University,Beijing,China 5.Institute of Advanced Biotechnology and School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 6.Center for Advanced Bioindustry Technologies,Chinese Academy of Agricultural Sciences,Beijing,China 7.Frontiers Science Center for Molecular Design Breeding,China Agricultural University,Beijing,China 8.Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing,China 9.Sanya Institute of China Agricultural University,Sanya,China 10.Hainan Yazhou Bay Seed Laboratory,Sanya,China |
| Recommended Citation GB/T 7714 |
Li，Yingnan,Huang，Boyu,Chen，Jian,et al. Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system[J]. Plant Biotechnology Journal,2023.
|
| APA |
Li，Yingnan.,Huang，Boyu.,Chen，Jian.,Huang，Liangliang.,Xu，Jianghai.,...&Lai，Jinsheng.(2023).Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system.Plant Biotechnology Journal.
|
| MLA |
Li，Yingnan,et al."Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system".Plant Biotechnology Journal (2023).
|
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