The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection

Yang，Qianting1,2; Qi，Furong1; Ye，Taosheng2,3; Li，Jinpei2,3; Xu，Gang1; He，Xiaomeng1; Deng，Guofang2,4; Zhang，Peize2,4; Liao，Mingfeng1,2; Qiao，Kun2,5; Zhang，Zheng1,2

2023

10.1080/22221751.2023.2239940

Emerging Microbes and Infections   Impact Factor & Quartile

2222-1751

Mycobacterium tuberculosis (Mtb) infection, including active tuberculosis (TB) and latent Mtb infection (LTBI), leads to diverse outcomes owing to different host immune responses. However, the immune mechanisms that govern the progression from LTBI to TB remain poorly defined in humans. Here, we profiled the lung immune cell populations within the bronchoalveolar lavage fluid (BALF) from patients with LTBI or TB using single-cell RNA sequencing (scRNA-seq). We found that Mtb infection substantially changed the immune cell compartments in the BALF, especially for the three subsets of macrophages, monocyte macrophage (MM)-CCL23, MM-FCN1, and MM-SPP1, which were found to be associated with the disease status of TB infection. Notably, MM-CCL23 cells derived from monocytes after stimulation with Mtb were characterized by high levels of chemokine (CCL23 and CXCL5) production and might serve as a marker for Mtb infection. The MM-CCL23 population mainly recruited CD8-CCR6 T cells through CCL20/CCR6, which was a prominent feature associated with protection immunity in LTBI. This study improves our understanding of the lung immune landscape during Mtb infection, which may inform future vaccine design for protective immunity.

CD8T cell Immunoregulation Macrophage Single-cell RNA sequencing Tuberculosis

SCI

English

First ; Corresponding

National Natural Science Foundation of China[82172286];National Natural Science Foundation of China[82200013];Shenzhen Science and Technology Innovation Program[ZDSYS20210623091810030];

Immunology ; Infectious Diseases ; Microbiology

Immunology ; Infectious Diseases ; Microbiology

WOS:001041399000001

TAYLOR & FRANCIS LTD

2-s2.0-85166387775

Scopus

1.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People’s Hospital; The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,China
2.Shenzhen Clinical Research Center for Tuberculosis,Shenzhen,China
3.Department of Respiratory endoscopy,Shenzhen Third People’s Hospital,Shenzhen,China
4.Department of Pulmonary Medicine & Tuberculosis,Shenzhen Third People’s Hospital,Shenzhen,China
5.Department of Thoracic Surgery,Shenzhen Third People’s Hospital,Shenzhen,China

Yang，Qianting,Qi，Furong,Ye，Taosheng,et al. The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection[J]. Emerging Microbes and Infections,2023,12(2).

Yang，Qianting.,Qi，Furong.,Ye，Taosheng.,Li，Jinpei.,Xu，Gang.,...&Zhang，Zheng.(2023).The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection.Emerging Microbes and Infections,12(2).

Yang，Qianting,et al."The interaction of macrophages and CD8 T cells in bronchoalveolar lavage fluid is associated with latent tuberculosis infection".Emerging Microbes and Infections 12.2(2023).