A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films

Yadid，Moran1,2; Hagel，Mario3; Labro，Megan Beldjilali3; Le-Roi，Baptiste3; Flaxer，Carina3; Flaxer，Eli4; Barnea，A. Ronny3; Tejman-Yarden，Shai5,6,7; Silberman，Eric2; Li，Xin8; Rauti，Rossana9; Leichtmann-Bardoogo，Yael3; Yuan，Hongyan8; Maoz，Ben M.3,10,11

2023

10.1002/advs.202207498

Advanced Science   Impact Factor & Quartile

2198-3844

Despite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length-based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility.

afterload cardiac in vitro models force application on cells magnetic gels preload

SCI

English

Others

Israel Science Foundation (ISF)[2248/19] ; Israel Ministry of Science and Technology[317351] ; Science, Technology, and Innovation Commission of Shenzhen Municipality[ZDSYS20210623092005017]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001033719100001

WILEY

2-s2.0-85165470619

Scopus

1.The Azrieli Faculty of Medicine,Bar Ilan University,Safed,8 Henrietta Szold St.,1311502,Israel
2.The Shmunis School of Biomedicine and Cancer Research,Tel Aviv University,Tel Aviv,69978,Israel
3.Department of Biomedical Engineering,Tel Aviv University,Tel Aviv,69978,Israel
4.AFEKA – Tel-Aviv Academic College of Engineering,Tel-Aviv,69107,Israel
5.The Edmond J. Safra International Congenital Heart Center,Sheba Medical Center,Ramat Gan,52621,Israel
6.The Engineering Medical Research Lab,Sheba Medical Center,Ramat Gan,52621,Israel
7.The Sackler School of Medicine,Tel Aviv University,Tel Aviv,69978,Israel
8.Shenzhen Key Laboratory of Soft Mechanics and Smart Manufacturing,Department of Mechanics and Aerospace Engineering,Southern University of Science and Technology,Shenzhen,518055,China
9.Department of Biomolecular Sciences,University of Urbino Carlo Bo,Urbino,61029,Italy
10.Sagol School of Neuroscience,Tel Aviv University,Tel Aviv,69978,Israel
11.The Center for Nanoscience and Nanotechnology,Tel Aviv University,Tel Aviv,69978,Israel

Yadid，Moran,Hagel，Mario,Labro，Megan Beldjilali,et al. A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films[J]. Advanced Science,2023.

Yadid，Moran.,Hagel，Mario.,Labro，Megan Beldjilali.,Le-Roi，Baptiste.,Flaxer，Carina.,...&Maoz，Ben M..(2023).A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films.Advanced Science.

Yadid，Moran,et al."A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films".Advanced Science (2023).