Histone variant HTB4 delays leaf senescence by epigenetic control of Ib bHLH transcription factor-mediated iron homeostasis
Leaf senescence is an orderly process regulated by multiple internal factors and diverse environmental stresses including nutrient deficiency. Histone variants are involved in regulating plant growth and development. However, their functions and underlying regulatory mechanisms in leaf senescence remain largely unclear. Here, we found that H2B histone variant HTB4 functions as a negative regulator of leaf senescence. Loss of function of HTB4 led to early leaf senescence phenotypes that were rescued by functional complementation. RNA-seq analysis revealed that several Ib subgroup basic helix–loop–helix (bHLH) transcription factors (TFs) involved in iron (Fe) homeostasis, including bHLH038, bHLH039, bHLH100, and bHLH101, were suppressed in the htb4 mutant, thereby compromising the expressions of FERRIC REDUCTION OXIDASE 2 (FRO2) and IRON-REGULATED TRANSPORTER (IRT1), two important components of the Fe uptake machinery. Chromatin immunoprecipitation-quantitative polymerase chain reaction analysis revealed that HTB4 could bind to the promoter regions of Ib bHLH TFs and enhance their expression by promoting the enrichment of the active mark H3K4me3 near their transcriptional start sites. Moreover, overexpression of Ib bHLH TFs or IRT1 suppressed the premature senescence phenotype of the htb4 mutant. Our work established a signaling pathway, HTB4-bHLH TFs-FRO2/IRT1-Fe homeostasis, which regulates the onset and progression of leaf senescence.
National Natural Science Foundation of China (NSFC)["31970196","32011540381","32170345"] ; Beijing Municipal Natural Science Foundation ; National Research Foundation of Korea (NRF)[2020K2A9A2A06051940] ; National Key Research and Development Program of China[2019YFA0903904]
|WOS Research Area|
|WOS Accession No|
|ESI Research Field|
PLANT & ANIMAL SCIENCE
Cited Times [WOS]:1
|Document Type||Journal Article|
|Department||Department of Biology|
1.State Key Laboratory of Tree Genetics and Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing,100083,China
2.Department of New Biology,Daegu Gyeongbuk Institute of Science and Technology (DGIST),Daegu,42988,South Korea
3.New Biology Research Center,Daegu Gyeongbuk Institute of Science and Technology (DGIST),Daegu,42988,South Korea
4.Department of Biology,Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Southern University of Science and Technology (SUSTech),Shenzhen,Guangdong,518055,China
Yang，Qi,Wang，Ting,Cao，Jie,et al. Histone variant HTB4 delays leaf senescence by epigenetic control of Ib bHLH transcription factor-mediated iron homeostasis[J]. New Phytologist,2023,240(2).
Yang，Qi.,Wang，Ting.,Cao，Jie.,Wang，Hou Ling.,Tan，Shuya.,...&Li，Zhonghai.(2023).Histone variant HTB4 delays leaf senescence by epigenetic control of Ib bHLH transcription factor-mediated iron homeostasis.New Phytologist,240(2).
Yang，Qi,et al."Histone variant HTB4 delays leaf senescence by epigenetic control of Ib bHLH transcription factor-mediated iron homeostasis".New Phytologist 240.2(2023).
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