Liver-targeting MRI contrast agent based on galactose functionalized o-carboxymethyl chitosan

Xu，Li1; Ren，Zhanying2,3; Li，Guolin4; Xu，Danni1; Miao，Jiaqian1; Ju，Jingxuan1; Mo，Xuan1; Wang，Xianghui2; Deng，Hongping1; Xu，Min2

2023

10.3389/fbioe.2023.1134665

Frontiers in Bioengineering and Biotechnology   Impact Factor & Quartile

2296-4185

2296-4185

Commercial gadolinium (Gd)-based contrast agents (GBCAs) play important role in clinical diagnostic of hepatocellular carcinoma, but their diagnostic efficacy remained improved. As small molecules, the imaging contrast and window of GBCAs is limited by low liver targeting and retention. Herein, we developed a liver-targeting gadolinium (Ⅲ) chelated macromolecular MRI contrast agent based on galactose functionalized o-carboxymethyl chitosan, namely, CS-Ga-(Gd-DTPA), to improve hepatocyte uptake and liver retention. Compared to Gd-DTPA and non-specific macromolecular agent CS-(Gd-DTPA), CS-Ga-(Gd-DTPA) showed higher hepatocyte uptake, excellent cell and blood biocompatibility in vitro. Furthermore, CS-Ga-(Gd-DTPA) also exhibited higher relaxivity in vitro, prolonged retention and better T1-weighted signal enhancement in liver. At 10 days post-injection of CS-Ga-(Gd-DTPA) at a dose of 0.03 mM Gd/Kg, Gd had a little accumulation in liver with no liver function damage. The good performance of CS-Ga-(Gd-DTPA) gives great confidence in developing liver-specifc MRI contrast agents for clinical translation.

biocompatibility gadolinium (Gd)-based MRI contrast agents galactose functionized o-carboxymethyl chitosan imaging contrast liver-targeting

SCI

English

Others

National Natural Science Foundation of China["82204505","21875068"] ; Natural Science Foundation of Shanghai[19ZR1438300]

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Multidisciplinary Sciences

WOS:001002579300001

FRONTIERS MEDIA SA

2-s2.0-85161000783

Scopus

1.Shanghai Frontiers Science Center of TCM Chemical Biology,Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai,China
2.Shanghai Key Laboratory of Magnetic Resonance,School of Physics and Electronic Science,East China Normal University,Shanghai,China
3.Southern University of Science and Technology,Shenzhen,China
4.Department of Stomatology,Shanghai 8th People’s Hospital,Shanghai,China

Xu，Li,Ren，Zhanying,Li，Guolin,et al. Liver-targeting MRI contrast agent based on galactose functionalized o-carboxymethyl chitosan[J]. Frontiers in Bioengineering and Biotechnology,2023,11.

Xu，Li.,Ren，Zhanying.,Li，Guolin.,Xu，Danni.,Miao，Jiaqian.,...&Xu，Min.(2023).Liver-targeting MRI contrast agent based on galactose functionalized o-carboxymethyl chitosan.Frontiers in Bioengineering and Biotechnology,11.

Xu，Li,et al."Liver-targeting MRI contrast agent based on galactose functionalized o-carboxymethyl chitosan".Frontiers in Bioengineering and Biotechnology 11(2023).