Genetic Code Expansion in Mammalian Cells
The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing such a unique handle into the protein of interest (POI), bioorthogonal reactions can be utilized in live cells to monitor or manipulate the interaction, translocation, function, and modification of the POI. Here, we describe a basic protocol outlining the necessary steps to incorporate a ncAA into a POI in mammalian cells.
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|Department||Department of Chemistry|
Department of Chemistry,Southern University of Science and Technology,Shenzhen,China
|First Author Affilication||Department of Chemistry|
|Corresponding Author Affilication||Department of Chemistry|
Wu，Zhigang,Wang，Jie. Genetic Code Expansion in Mammalian Cells. 2023-01-01.
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