中文版 | English
Title

Genetic Code Expansion in Mammalian Cells

Author
Publication Years
2023
Keywords
Language
English
URL[Source Record]
Abstract
The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing such a unique handle into the protein of interest (POI), bioorthogonal reactions can be utilized in live cells to monitor or manipulate the interaction, translocation, function, and modification of the POI. Here, we describe a basic protocol outlining the necessary steps to incorporate a ncAA into a POI in mammalian cells.
DOI
Source Title
Volume
2676
Pages
159-167
ISSN
1064-3745
SUSTech Authorship
First ; Corresponding
Scopus EID
2-s2.0-85160979030
Data Source
Scopus
Corresponding AuthorWang,Jie
EISSN
1940-6029
Citation statistics
Cited Times [WOS]:0
Document TypeOther
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/560303
DepartmentDepartment of Chemistry
Affiliation
Department of Chemistry,Southern University of Science and Technology,Shenzhen,China
First Author AffilicationDepartment of Chemistry
Corresponding Author AffilicationDepartment of Chemistry
Recommended Citation
GB/T 7714
Wu,Zhigang,Wang,Jie. Genetic Code Expansion in Mammalian Cells. 2023-01-01.
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