Diversity and salinity adaptations of ammonia oxidizing archaea in three estuaries of China

Zou, Dayu1,2; Chen, Jianfang3; Zhang, Chuanlun4,5; Kao, Shuh-Ji6; Liu, Hongbin7,8; Li, Meng1,2

2023-09-01

10.1007/s00253-023-12761-4

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   Impact Factor & Quartile

0175-7598

1432-0614

Ammonia-oxidizing archaea (AOA) are ubiquitously found in diverse habitats and play pivotal roles in the nitrogen and carbon cycle, especially in estuarine and coastal environments. Despite the fact that the diversity and distribution of AOA are thought to be tightly linked to habitats, little is known about the relationship that underpins their genomic traits, adaptive potentials, and ecological niches. Here, we have characterized and compared the AOA community in three estuaries of China using metagenomics. AOA were the dominant ammonia oxidizers in the three estuaries. Through phylogenetic analyses, five major AOA groups were identified, including the Nitrosomarinus-like, Nitrosopumilus-like, Aestuariumsis-like, Nitrosarchaeum-like, and Nitrosopelagicus-like groups. Statistical analyses showed that the aquatic and sedimentary AOA communities were mainly influenced by spatial factors (latitude and water depth) and environmental factors (salinity, pH, and dissolved oxygen) in estuaries, respectively. Compared to AOA dwelling in terrestrial and marine habitats, estuarine AOA encoded more genes involved in glucose and amino acid metabolism, transport systems, osmotic control, and cell motility. The low proteome isoelectric points (pI), high content of acidic amino acids, and the presence of potassium ion and mechanosensitive channels suggest a "salt-in" strategy for estuarine AOA to counteract high osmolarity in their surroundings. Our findings have indicated potential adaptation strategies and highlighted their importance in the estuarine nitrogen and carbon cycles.

Ammonia oxidizing archaea Metagenomics Adaptation Estuaries

SCI

English

Others

National Natural Science Foundation of China["32225003","31970105","992251306","42141003"] ; Shenzhen Science and Technology Program[JCYJ20200109105010363] ; Innovation Team Project of Universities in Guangdong Province[2020KCXTD023] ; Shenzhen University 2035 Program for Excellent Research[2022B002] ; China Postdoctoral Science Foundation[2022M722175] ; Long Term Observation and Research Plan in the Changjiang Estuary and the Adjacent East China Sea Project (LORCE)[14282] ; Department of Science and Technology of Guangdong Province[2021B151512008] ; Shenzhen Key Laboratory of Marine Archaea GeoOmics, Southern University of Science and Technology[ZDSYS201802081843490] ; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[K19313901] ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL20SC01]

Biotechnology & Applied Microbiology

Biotechnology & Applied Microbiology

WOS:001065379600001

SPRINGER

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Shenzhen Univ, Inst Adv Study, Archaeal Biol Ctr, Shenzhen 518060, Peoples R China
2.Shenzhen Univ, Inst Adv Study, Shenzhen Key Lab Marine Microbiome Engn, Shenzhen 518060, Peoples R China
3.Minist Nat Resources, Inst Oceanog 2, Key Lab Marine Ecosyst Dynam, Hangzhou 310012, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Key Lab Marine Archaea Geoom, Shenzhen 518000, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou 510000, Peoples R China
6.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361000, Peoples R China
7.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China
8.Hong Kong Univ Sci & Technol, Hong Kong Branch, Southern Marine Sci & Engn Guangdong Lab Guangzhou, Hong Kong, Peoples R China

Zou, Dayu,Chen, Jianfang,Zhang, Chuanlun,et al. Diversity and salinity adaptations of ammonia oxidizing archaea in three estuaries of China[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2023.

Zou, Dayu,Chen, Jianfang,Zhang, Chuanlun,Kao, Shuh-Ji,Liu, Hongbin,&Li, Meng.(2023).Diversity and salinity adaptations of ammonia oxidizing archaea in three estuaries of China.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY.

Zou, Dayu,et al."Diversity and salinity adaptations of ammonia oxidizing archaea in three estuaries of China".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2023).