Reverse flow enhanced inertia pinched flow fractionation

Wang, Saijie1; Xu, Quanchen1; Zhang, Zhihan1; Chen, Shengbo1; Jiang, Yizhou1,2; Feng, Zhuowei1; Wang, Dou1; Jiang, Xingyu1

2023-09-01

10.1039/d3lc00473

LAB ON A CHIP   Impact Factor & Quartile

1473-0197

1473-0189

["Particle separation plays a critical role in many biochemical analyses. In this article, we report a method of reverse flow enhanced inertia pinched flow fractionation (RF-iPFF) for particle separation. RF-iPFF separates particles by size based on the flow-induced inertial lift, and in the abruptly broadened segment, reverse flow is utilized to further enhance the separation distance between particles of different sizes. The separation performance can be significantly improved by reverse flow. Generally, compared with the case without reverse flow, this RF-iPFF technique can increase the particle throughput by about 10 times. To demonstrate the advantages of RF-iPFF, RF-iPFF was compared with traditional iPFF through a control experiment. RF-iPFF consistently outperformed iPFF across various conditions we studied. In addition, we use tumor cells spiked into the human whole blood to evaluate the separation performance of RF-iPFF.","By setting the reverse flow channel at the end of the abruptly broadened segment, the flow field can be fundamentally changed, and the separation performance can be improved comprehensively compared with the traditional iPFF device."]

SCI

English

First ; Corresponding

National Key R&D Program of China["2021YFF1200100","2022YFC2403500","2020YFA0908900","2018YFA0902600","2022YFB3804700"] ; National Natural Science Foundation of China["22234004","81730051","22204068"] ; Shenzhen Science and Technology Program["JCYJ20210324105006017","KQTD20190929172743294","JSGG20220606142200001","JSGG20220919091201003","KCXFZ20211020163544002","JCYJ20200109141231365"] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Natural Science Foundation of Shenzhen City[JCYJ20190809115005628] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; Guangdong Major Talent Introduction Project[2019CX01Y196] ; Guangdong Basic and Applied Basic Research Foundation["2019A1515110292","2021A1515220111"] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:001064612300001

ROYAL SOC CHEMISTRY

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
2.Chinese Acad Med Sci & Peking Union Med Coll, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing 100037, Peoples R China

Wang, Saijie,Xu, Quanchen,Zhang, Zhihan,et al. Reverse flow enhanced inertia pinched flow fractionation[J]. LAB ON A CHIP,2023.

Wang, Saijie.,Xu, Quanchen.,Zhang, Zhihan.,Chen, Shengbo.,Jiang, Yizhou.,...&Jiang, Xingyu.(2023).Reverse flow enhanced inertia pinched flow fractionation.LAB ON A CHIP.

Wang, Saijie,et al."Reverse flow enhanced inertia pinched flow fractionation".LAB ON A CHIP (2023).