Differential regulation of hippocampal transcriptome by circulating estrogen

Iqbal, Javed1,2,3; Bibi, Maryam4; Huang, Geng-Di1,2,3; Xue, Yan-Xue5,6; Khatttak, Jabbar Zaman Khan4; Yang, Mei2,3,7,8; Jia, Xiao-Jian2,3,7,8

2023-12-01

10.1007/s10142-023-01234-6

FUNCTIONAL & INTEGRATIVE GENOMICS   Impact Factor & Quartile

1438-793X

1438-7948

Estrogen (E2) modulates the synaptic structure and plasticity in the hippocampus. Previous studies showed that E2 fluctuations during various phases of the menstrual cycle produce subtle neurosynaptic changes that impact women's behavior, emotion, and cognitive functions. In this study, we explored the transcriptome of the hippocampus via RNA-seq (RNA-sequencing) between proestrus (PE) and diestrus (DE) stages in young female rats to determine the effect of E2 of PE and DE stages on hippocampal gene expression. We identified 238 genes (at 1.5-fold-change selection criteria, FDR adjusted p-value < 0.05) as differentially expressed genes (DEGs) that responded to E2 between PE and DE stages. Functional analysis based on Gene Ontology (GO) revealed that a higher E2 level corresponded to an increase in gene transcription among most of the DEGs, suggesting biological mechanisms operating differentially in the hippocampus of female rats between PE and DE stages in the estrus cycle; while analysis with Kyoto Encyclopedia of Genes and Genomes database (KEGG) found that the DEGs involving neuroactive ligand-receptor interaction, antigen processing, cell adhesion molecules, and presentation were upregulated in PE stage, whereas DEGs in pathways relating to bile secretion, coagulation cascades, osteoclast differentiation, cysteine and methionine metabolism were upregulated in DE stage of the estrus cycle. The high-fold expression of DEGs was confirmed by a follow-up quantitative real-time PCR. Our findings in this current study have provided fundamental information for further dissection of neuro-molecular mechanisms in the hippocampus in response to E2 fluctuation and its relationship with disorders.

Estrogen Spines Hippocampus Estrous cycle

SCI

English

Corresponding

Genetics & Heredity

Genetics & Heredity

WOS:001070903100001

SPRINGER HEIDELBERG

Web of Science

1.Peking Univ, Shenzhen Grad Sch, Shenzhen, Peoples R China
2.Shenzhen Kangning Hosp, Shenzhen Clin Res Ctr Mental Disorders, Shenzhen Engn Res Ctr Precis Psychiat Technol, Dept Addict Med, 77 Zhenbi Rd, Shenzhen 518118, Guangdong, Peoples R China
3.Shenzhen Mental Hlth Ctr, 77 Zhenbi Rd, Shenzhen 518118, Guangdong, Peoples R China
4.Int Islamic Univ, Dept Biol Sci, Islamabad, Pakistan
5.Peking Univ, Natl Inst Drug Dependence, Beijing, Peoples R China
6.Peking Univ, Beijing Key Lab Drug Dependence, Beijing, Peoples R China
7.Shenzhen Univ, Clin Coll Mental Hlth, Hlth Sci Ctr, Shenzhen, Peoples R China
8.Southern Univ Sci & Technol, Affiliated Mental Hlth Ctr, Shenzhen, Peoples R China

Iqbal, Javed,Bibi, Maryam,Huang, Geng-Di,et al. Differential regulation of hippocampal transcriptome by circulating estrogen[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2023,23(4).

Iqbal, Javed.,Bibi, Maryam.,Huang, Geng-Di.,Xue, Yan-Xue.,Khatttak, Jabbar Zaman Khan.,...&Jia, Xiao-Jian.(2023).Differential regulation of hippocampal transcriptome by circulating estrogen.FUNCTIONAL & INTEGRATIVE GENOMICS,23(4).

Iqbal, Javed,et al."Differential regulation of hippocampal transcriptome by circulating estrogen".FUNCTIONAL & INTEGRATIVE GENOMICS 23.4(2023).