Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair

Wang, Ziyan1; Mi, Feixue1; Li, Jinchen1; Chen, Dandan2; Lin, Minmin1; Wang, Xinyu1; Wu, Siying1; Wu, Changfeng1; Liu, Chao1,3

2023-09-21

10.1021/acsbiomaterials.3c00575

ACS BIOMATERIALS SCIENCE & ENGINEERING   Impact Factor & Quartile

2373-9878

The use of bone marrow stromal cells (BMSCs) for bone defect repair has shown great promise due to their differentiation potential. However, isolating the BMSCs from various cell types within the bone marrow remains challenging. To tackle this issue, we utilized semiconducting polymer dots (Pdots) as markers to select the BMSCs within a specific time frame. The therapeutic efficacy of the obtained Pdot-labeled BMSCs was assessed in a bone defect model. Initially, we evaluated the binding capacity of the Pdots with four different types of cells present in the bone marrow including BMSCs, osteoblasts, macrophages, and vascular endothelial cells, in vitro. Notably, BMSCs showed the most rapid uptake of the Pdots, being labeled within only one h of coculture, while other cells took four h to become labeled. Moreover, by colocalizing the Pdots with Prrx1, Sca-1, OSX, F480, and CD105 in the bone marrow cells of monocortical tibial defect (MTD) mice in vivo, we determined the proportions of BMSCs, macrophages, and vascular endothelial cells among all labeled cells from 1 to 8 h after the Pdots injection. It was found that BMSCs have the highest proportion (92%) among all labeled cells extracted after 1 h of Pdots injection. The therapeutic efficacy of the obtained Pdots-labeled BMSCs (1 h) was assessed in a bone defect model. Results showed that the new bone accrual was significantly increased in the treatment of Pdots-labeled BMSCs compared to the bone marrow cell-treated group. Our study revealed that BMSCs screened by the Pdots could improve bone defect repair, suggesting a promising application of the Pdots in bone healing.

bone marrow stromal cell semiconducting polymer dots cell therapy bone marrow transplantation bone regeneration

SCI

English

First ; Corresponding

Shenzhen Science and Technology Innovation Commission["KQTD20200820113012029","20201022173046002","JCYJ20190809114209434"]

Materials Science

Materials Science, Biomaterials

WOS:001071380100001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Qingdao Univ, Coll Chem & Chem Engn, Qingdao 266071, Shandong, Peoples R China
3.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China

Wang, Ziyan,Mi, Feixue,Li, Jinchen,et al. Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2023,9(10).

Wang, Ziyan.,Mi, Feixue.,Li, Jinchen.,Chen, Dandan.,Lin, Minmin.,...&Liu, Chao.(2023).Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair.ACS BIOMATERIALS SCIENCE & ENGINEERING,9(10).

Wang, Ziyan,et al."Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair".ACS BIOMATERIALS SCIENCE & ENGINEERING 9.10(2023).