Single-cell RNA sequencing reveals the effects of capsaicin in the treatment of sepsis-induced liver injury

Zhang, Qian1,2,3,4,5; Liu, Jing1,2; Shen, Jing6; Ou, Jinhuan1,2; Wong, Yin Kwan7; Xie, Lulin1,2; Huang, Jingnan1,2; Zhang, Chunting1,2; Fu, Chunjin1,2; Chen, Junhui1,2; Chen, Jiayun5; He, Xueling5; Shi, Fei8; Luo, Piao1,2,3,5; Gong, Ping9; Liu, Xueyan1,2; Wang, Jigang1,2,3,4,5

2023-10-01

10.1002/mco2.395

MEDCOMM   Impact Factor & Quartile

2688-2663

["Sepsis is a difficult-to-treat systemic condition in which liver dysfunction acts as both regulator and target. However, the dynamic response of diverse intrahepatic cells to sepsis remains poorly characterized. Capsaicin (CAP), a multifunctional chemical derived from chilli peppers, has recently been shown to potentially possess anti-inflammatory effects, which is also one of the main approaches for drug discovery against sepsis. We performed single-cell RNA transcriptome sequencing on 86,830 intrahepatic cells isolated from normal mice, cecal ligation and puncture-induced sepsis model mice and CAP-treated mice. The transcriptional atlas of these cells revealed dynamic changes in hepatocytes, macrophages, neutrophils, and endothelial cells in response to sepsis. Among the extensive crosstalk across these major subtypes, KC_Cxcl10 shared strong potential interaction with other cells when responding to sepsis. CAP mitigated the severity of inflammation by partly reversing these pathophysiologic processes. Specific cell subpopulations in the liver act collectively to escalate inflammation, ultimately causing liver dysfunction. CAP displays its health-promoting function by ameliorating liver dysfunction induced by sepsis. Our study provides valuable insights into the pathophysiology of sepsis and suggestions for future therapeutic gain.","Single-cell transcriptome reveals the protective mechanism of capsaicin against sepsis induced liver injury in mice. The transcriptional atlas of cells revealed dynamic changes in hepatocytes (Hep), macrophages, neutrophils (Neu), and endothelial cells (Endo) in response to sepsis. Among the extensive crosstalk across these major subtypes, KC_Cxcl10 shared strong potential interaction with other cells when responding to sepsis.image"]

capsaicin inflammation scRNA-seq sepsis

ESCI

English

First ; Corresponding

The study was supported by the Shenzhen Key Laboratory of Prevention and Treatment of Severe Infections (ZDSYS20200811142804014); Shenzhen Clinical Medical Research Center for Geriatric Diseases, the Science and Technology Foundation of Shenzhen (JCYJ20210[ZDSYS20200811142804014] ; Shenzhen Clinical Medical Research Center for Geriatric Diseases[JCYJ20210324115800001] ; Science and Technology Foundation of Shenzhen["2020YFA0908000","2022YFC2303600"] ; National Key Research and Development Program of China["JCYJ20200109120205924","RCYX20221008092950121"] ; Shenzhen Science and Technology Innovation Commission[ZYYCXTD-C-202002] ; Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[SZXK046]

Research & Experimental Medicine

Medicine, Research & Experimental

WOS:001076107600001

WILEY

Web of Science

1.Southern Univ Sci & Technol, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Crit Med,Affiliated Hosp 1,Jinan Univ, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Clin Med Coll 2, Shenzhen Peoples Hosp, Shenzhen Clin Res Ctr Geriatr,Affiliated Hosp 1,Ji, Shenzhen 518020, Guangdong, Peoples R China
3.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou, Guangdong, Peoples R China
4.Southern Med Univ, Inst Basic Integrat Med, Sch Tradit Chinese Med, Guangzhou, Guangdong, Peoples R China
5.China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing, Peoples R China
6.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Oncol, Shenzhen, Guangdong, Peoples R China
7.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore, Singapore
8.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Infect Dis, Shenzhen, Guangdong, Peoples R China
9.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Emergency, Shenzhen, Guangdong, Peoples R China

Zhang, Qian,Liu, Jing,Shen, Jing,et al. Single-cell RNA sequencing reveals the effects of capsaicin in the treatment of sepsis-induced liver injury[J]. MEDCOMM,2023,4(5).

Zhang, Qian.,Liu, Jing.,Shen, Jing.,Ou, Jinhuan.,Wong, Yin Kwan.,...&Wang, Jigang.(2023).Single-cell RNA sequencing reveals the effects of capsaicin in the treatment of sepsis-induced liver injury.MEDCOMM,4(5).

Zhang, Qian,et al."Single-cell RNA sequencing reveals the effects of capsaicin in the treatment of sepsis-induced liver injury".MEDCOMM 4.5(2023).