Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device

Liu, Ying1; Yang, Yang2; Wang, Guanghui1; Wang, Dou1; Shao, Pan-Lin3,4,5; Tang, Jiahu1; He, Tingzhen1; Zheng, Jintao1; Hu, Ruibin1; Liu, Yiyi1; Xu, Ziyi1; Niu, Dan1; Lv, Jiahui1; Yang, Jingkai1; Xiao, Hongjun1; Wu, Shuai2; He, Shuang1; Tang, Zhongrong1; Liu, Yan2; Tang, Meijie6; Jiang, Xingyu1; Yuan, Jing2; Dai, Hongjie7; Zhang, Bo1

2023-09-01

10.1038/s41551-023-01092-4

NATURE BIOMEDICAL ENGINEERING   Impact Factor & Quartile

2157-846X

["Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.","A portable multiplexed assay allows for the rapid detection, via plasmonic-gold-enhanced near-infrared fluorescence, of SARS-CoV-2 RNA at single-copy sensitivity and single-nucleotide specificity for the discrimination of variants of the virus."]

SCI

English

First ; Corresponding

National Natural Science Foundation of China["22274069","22304070"] ; Guangdong Basic and Applied Basic Research Foundation[2022A1515011408] ; Shenzhen Science and Technology programme project["CYJ20180504165657443","JCYJ20210324104007020"]

Engineering

Engineering, Biomedical

WOS:001068142600001

NATURE PORTFOLIO

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Infect Dis Dept,Hosp 2,State Key Discipline Infect, Shenzhen, Peoples R China
3.Guangzhou Med Univ, Guangzhou Municipal & Guangdong Prov Key Lab Mol T, NMPA, Guangzhou, Peoples R China
4.Guangzhou Med Univ, Sch Pharmaceut Sci, State Key Lab Resp Dis, Guangzhou, Peoples R China
5.Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou, Peoples R China
6.Nirmidas Biotech Inc, Palo Alto, CA 94043 USA
7.Stanford Univ, Dept Chem, Stanford, CA 94305 USA

Liu, Ying,Yang, Yang,Wang, Guanghui,et al. Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device[J]. NATURE BIOMEDICAL ENGINEERING,2023.

Liu, Ying.,Yang, Yang.,Wang, Guanghui.,Wang, Dou.,Shao, Pan-Lin.,...&Zhang, Bo.(2023).Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device.NATURE BIOMEDICAL ENGINEERING.

Liu, Ying,et al."Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device".NATURE BIOMEDICAL ENGINEERING (2023).