Membrane-enclosed Pseudomonas quinolone signal attenuates bacterial virulence by interfering with quorum sensing

Li, Xia1; Wang, Gerun1; Guo, Quan1; Cui, Binbin1; Wang, Mingfang1; Song, Shihao1,2; Yang, Liang3; Deng, Yinyue1,2

2023-10-05

10.1128/aem.01184-23

APPLIED AND ENVIRONMENTAL MICROBIOLOGY   Impact Factor & Quartile

0099-2240

1098-5336

Outer membrane vesicle (OMV)-delivered Pseudomonas quinolone signal (PQS) plays a critical role in cell-cell communication in Pseudomonas aeruginosa. However, the functions and mechanisms of membrane-enclosed PQS in interspecies communication in microbial communities are not clear. Here, we demonstrate that PQS delivered by both OMVs from P. aeruginosa and liposome reduces the competitiveness of Burkholderia cenocepacia, which usually shares the same niche in the lungs of cystic fibrosis patients, by interfering with quorum sensing (QS) in B. cenocepacia through the LysR-type regulator ShvR. Intriguingly, we found that ShvR regulates the production of the QS signals cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) by directly binding to the promoters of signal synthase-encoding genes. Perception of PQS influences the regulatory activity of ShvR and thus ultimately reduces QS signal production and virulence in B. cenocepacia. Our findings provide insights into the interspecies communication mediated by the membrane-enclosed QS signal among bacterial species residing in the same microbial community.

Burkholderia cenocepacia quorum sensing LysR family transcriptional regulator outer membrane vesicle PQS signal

SCI

English

Others

This work was financially supported by the National Key Research and Development Program of China (2021YFA0717003) and the Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20200109142416497).[2021YFA0717003] ; National Key Research and Development Program of China[JCYJ20200109142416497]

Biotechnology & Applied Microbiology ; Microbiology

Biotechnology & Applied Microbiology ; Microbiology

WOS:001078417800001

AMER SOC MICROBIOLOGY

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen Campus Sun Yat Sen Univ, Shenzhen, Guangdong, Peoples R China
2.Hainan Univ, Sch Pharmaceut Sci, Haikou, Hainan, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen, Guangdong, Peoples R China

Li, Xia,Wang, Gerun,Guo, Quan,et al. Membrane-enclosed Pseudomonas quinolone signal attenuates bacterial virulence by interfering with quorum sensing[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2023.

Li, Xia.,Wang, Gerun.,Guo, Quan.,Cui, Binbin.,Wang, Mingfang.,...&Deng, Yinyue.(2023).Membrane-enclosed Pseudomonas quinolone signal attenuates bacterial virulence by interfering with quorum sensing.APPLIED AND ENVIRONMENTAL MICROBIOLOGY.

Li, Xia,et al."Membrane-enclosed Pseudomonas quinolone signal attenuates bacterial virulence by interfering with quorum sensing".APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2023).