Long-term assessment of cutaneous inflammation and treatment using optical resolution photoacoustic microscopy

Zhao, Yuanlong1; Li, Tingting1; Guo, Heng1; Hu, Rui2; Xi, Lei1,3

2023-09-01

10.1364/BOE.499627

BIOMEDICAL OPTICS EXPRESS   Impact Factor & Quartile

2156-7085

Cutaneous inflammation is an acute skin disease characterized by edema and vascular hyperplasia. Longitudinal monitoring of vasculature is crucial for studying the development of inflammation and evaluating the therapeutic efficacy of drugs. Optical-resolution photoacoustic microscopy (OR-PAM) is a hybrid imaging tool for non-invasive and label-free visualization of microcirculations with a capillary-scale spatial resolution. In this study, we assess the feasibility of OR-PAM for long-term monitoring of vascular changes in 12-O-Tetradecanoylphorbol-13-Acetate (TPA)-induced mouse models, as well as the corresponding treatment process. Quantitative vascular evaluation is conducted based on derived key parameters, including vessel length, branchpoint number, vessel area fraction, vessel diameter, fractal dimension, vessel tortuosity and ear thickness, which reveal that vascular morphological changes are highly dependent on the concentration of TPA and existence of therapeutic drugs. Furthermore, the results show the potential of OR-PAM in the clinical management of inflammation and as an effective tool to evaluate vascular responses to pharmacological interventions in vivo.(c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

SCI

English

First ; Corresponding

National Natural Science Foundation of China["62022037","61775028","81571722","61528401"] ; Guangdong Science and Technology Department["2019ZT08Y191","SZBL2020090501013","2022B1212010003"] ; Department of Education of Guangdong Province[2021ZDZX1064] ; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20200109141222892"] ; ; Startup grant from Southern University of Science and Technology[PDJH2021C008] ; Department of Education of Guangdong Province["62022037","61775028","81571722","61528401"] ; Shenzhen Science and Technology Program["2019ZT08Y191","SZBL2020090501013","2022B1212010003"] ; Startup grant from Southern University of Science and Technology[2021ZDZX1064]

Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging

Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging

WOS:001075528800005

Optica Publishing Group

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Shenzhen Univ, Coll Phys & Optoelect Engn, State Key Lab Radio Frequency Heterogeneous Integr, Key Lab Optoelect Devices & Syst,Minist Educ & Gua, Shenzhen 518060, Peoples R China
3.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China

Zhao, Yuanlong,Li, Tingting,Guo, Heng,et al. Long-term assessment of cutaneous inflammation and treatment using optical resolution photoacoustic microscopy[J]. BIOMEDICAL OPTICS EXPRESS,2023,14(9).

Zhao, Yuanlong,Li, Tingting,Guo, Heng,Hu, Rui,&Xi, Lei.(2023).Long-term assessment of cutaneous inflammation and treatment using optical resolution photoacoustic microscopy.BIOMEDICAL OPTICS EXPRESS,14(9).

Zhao, Yuanlong,et al."Long-term assessment of cutaneous inflammation and treatment using optical resolution photoacoustic microscopy".BIOMEDICAL OPTICS EXPRESS 14.9(2023).