A Mitochondria-Localized Iridium(III) Complex for Simultaneous Two-Photon Phosphorescence Lifetime Imaging of Downstream Products N₂O₃ and ONOO^- of Endogenous Nitric Oxide

Wu, Weijun1,2; Wen, Yuxin1; Chen, Yu1; Ji, Liangnian1; Chao, Hui1,3

2023-10-01

10.1021/acs.analchem.3c03023

ANALYTICAL CHEMISTRY   Impact Factor & Quartile

0003-2700

1520-6882

Nitric oxide (NO) serves as a ubiquitous and fundamental signaling molecule involved in intricate effects on both physiological and pathological processes. NO, biosynthesized by nitric oxide synthase (NOS) or generated from nitrite, can form nitrosation reagent N2O3 (4NO + O-2 = 2N(2)O(3)) through its oxidation or quickly produce peroxynitrite anion ONOO- (NO + center dot O-2(-) = ONOO-) by reacting with superoxide anion (center dot O-2(-)). However, most of the existing luminescent probes for NO just focus on specificity and utilize only a single signal to distinguish products N2O3 or ONOO-. In most of the present work, they differentiate one product from another simply by fluorescence signal or fluorescence intensity, which is not enough to distinguish accurately the behavior of NO in living cells. Herein, a new mitochondria-targeted and two-photon near-infrared (NIR) phosphorescent iridium(III) complex, known as Ir-NBD, has been designed for accurate detection and simultaneous imaging of two downstream products of endogenous NO, i.e., N2O3 and ONOO-.Ir-NBD exhibits a rapid response to N2O3 and ONOO- in enhanced phosphorescence intensity, increased phosphorescence lifetime, and an exceptionally high two-photon cross-section, reaching values of 78 and 85 GM, respectively, after the reaction. Furthermore, we employed multiple imaging methods, phosphorescence intensity imaging, and phosphorescence lifetime imaging together to image even distinguish N2O3 and ONOO- by probe Ir-NBD. Thus, coupled with its excellent photometrics, Ir-NBD enabled the detection of the basal level of intracellular NO accurately by responding to N2O3 and ONOO- in the lipopolysaccharide-stimulated macrophage model in virtue of fluorescence signal and phosphorescence lifetime imaging, revealing precisely the endogenous mitochondrial NO distribution during inflammation in a cell environment.

SCI

English

NI Journal Papers

Others

National Natural Science Foundation of China[2021RC5028] ; Science and Technology Innovation Program of Hunan Province of China[2021M691414]

Chemistry

Chemistry, Analytical

WOS:001092753800001

AMER CHEMICAL SOC

CHEMISTRY

Web of Science

1.Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
3.Hunan Univ Sci & Technol, Sch Chem & Chem Engn, MOE Key Lab Theoret Organ Chem & Funct Mol, Xiangtan 400201, Peoples R China

Wu, Weijun,Wen, Yuxin,Chen, Yu,et al. A Mitochondria-Localized Iridium(III) Complex for Simultaneous Two-Photon Phosphorescence Lifetime Imaging of Downstream Products N₂O₃ and ONOO^- of Endogenous Nitric Oxide[J]. ANALYTICAL CHEMISTRY,2023.

Wu, Weijun,Wen, Yuxin,Chen, Yu,Ji, Liangnian,&Chao, Hui.(2023).A Mitochondria-Localized Iridium(III) Complex for Simultaneous Two-Photon Phosphorescence Lifetime Imaging of Downstream Products N₂O₃ and ONOO^- of Endogenous Nitric Oxide.ANALYTICAL CHEMISTRY.

Wu, Weijun,et al."A Mitochondria-Localized Iridium(III) Complex for Simultaneous Two-Photon Phosphorescence Lifetime Imaging of Downstream Products N₂O₃ and ONOO^- of Endogenous Nitric Oxide".ANALYTICAL CHEMISTRY (2023).