An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development

Wang, Zeshuai1,2; Wu, Zhisheng1,3; Wang, Hao4; Feng, Ruoqing1; Wang, Guanlin5,6; Li, Muxi7; Wang, Shuang-Yin8; Chen, Xiaoyan4; Su, Yiyi1,2; Wang, Jun1; Zhang, Weiwen9; Bao, Yuzhou1,7; Lan, Zhenwei7; Song, Zhuo1,4; Wang, Yiheng1,10; Luo, Xianyang11; Zhao, Lingyu1,12; Hou, Anli13; Tian, Shuye14; Gao, Hongliang13; Miao, Wenbin13; Liu, Yingyu4; Wang, Huilin4; Yin, Cui9; Ji, Zhi-Liang7; Feng, Mingqian2; Liu, Hongkun15; Diao, Lianghui16; Amit, Ido8; Chen, Yun3,17; Zeng, Yong16; Ginhoux, Florent18,19,20,21; Wu, Xueqing9; Zhu, Yuanfang4; Li, Hanjie1

2023-09-28

10.1016/j.cell.2023.08.019

CELL   Impact Factor & Quartile

0092-8674

1097-4172

Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, func-tion, and specification remain inadequately understood during human development. We generated a single -cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4-26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage sub-types. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac -derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dy-namics of human macrophages and unravels their diverse functions during development.

SCI

English

NI Journal Papers

Others

National Key R&D Program of China[2020YFA0804100] ; National Natural Science Foundation of China[32170919] ; Shenzhen Institute of Synthetic Biology Scientific Research Program[JCHZ20210003] ; Shenzhen Science and Technology Program["KQTD20210811090115019","RCYX20210609104608036"] ; Shenzhen Key Medical Discipline Construction Fund[SZXK028] ; Fudan University Startup Grant[JIH2313032Y]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:001088848000001

CELL PRESS

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Key Lab Quantitat Engn Biol, Shenzhen 518055, Peoples R China
2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China
3.Southeast Univ, Sch Chem & Chem Engn, Nanjing, Peoples R China
4.Jinan Univ, Shenzhen Baoan Womens & Childrens Hosp, Maternal Fetal Med Inst, Dept Obstet & Gynaecol, Shenzhen, Peoples R China
5.Fudan Univ, Inst Metab & Integrat Biol, Ctr Evolutionary Biol, Shanghai Key Lab Metab Remodeling & Hlth, Shanghai, Peoples R China
6.Shanghai Qi Zhi Inst, Shanghai, Peoples R China
7.Xiamen Univ, Fac Med & Life Sci, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen, Peoples R China
8.Weizmann Inst Sci, Dept Syst Immunol, Rehovot, Israel
9.Shenzhen Univ, Shenzhen Univ Gen Hosp, Dept Gynaecol & Obstet, Shenzhen, Peoples R China
10.China Agr Univ, Coll Biol Sci, Beijing, Peoples R China
11.Chinese Acad Sci, Brain Cognit & Brain Dis Inst, Shenzhen Hong Kong Inst Brain Sci, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
12.Chinese Acad Med Sci & Peking Union Med Coll, Grad Sch, Beijing, Peoples R China
13.Univ Chinese Acad Sci, Shenzhen Hosp, Shenzhen, Peoples R China
14.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen 518055, Peoples R China
15.Jinxin Fertil Grp Ltd, Chengdu, Peoples R China
16.Shenzhen Zhongshan Urol Hosp, Shenzhen Zhongshan Inst Reprod & Genet, Shenzhen Key Lab Reprod Immunol Periimplantat, Shenzhen, Peoples R China
17.Nanjing Med Univ, Dept Immunol, Nanjing, Peoples R China
18.Shanghai Jiao Tong Univ, Shanghai Inst Immunol, Dept Immunol & Microbiol, Sch Med, Shanghai 200025, Peoples R China
19.Gustave Roussy Canc Campus, INSERM U1015, F-94800 Villejuif, France
20.ASTAR, Singapore Immunol Network SIgN, 8A Biomed Grove, Immunos, Singapore 138648, Singapore
21.SingHlth Duke NUS Acad Med Ctr, Translat Immunol Inst, Singapore 169856, Singapore

Wang, Zeshuai,Wu, Zhisheng,Wang, Hao,et al. An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development[J]. CELL,2023,186(20).

Wang, Zeshuai.,Wu, Zhisheng.,Wang, Hao.,Feng, Ruoqing.,Wang, Guanlin.,...&Li, Hanjie.(2023).An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development.CELL,186(20).

Wang, Zeshuai,et al."An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development".CELL 186.20(2023).