TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype

Karanovic, Una1; Lei, Lei1; Martens, Craig A.2; Barbian, Kent2; Mcclarty, Grant3; Caldwell, Harlan D.1; Yang, Chunfu4

2023-01-17

10.1093/femspd/ftad026

PATHOGENS AND DISEASE   Impact Factor & Quartile

2049-632X

Chlamydia trachomatis is an obligate intracellular bacterium that causes blinding trachoma and sexually transmitted disease. The chlamydial plasmid is a critical virulence factor in the pathogenesis of these diseases. Plasmid gene protein 4 (Pgp4) plays a major role in chlamydial virulence by regulating the expression of both chromosomal genes and Pgp3. Despite the importance of Pgp4 in mediating lytic exit from host cells the pathogenic mechanism by which it functions is unknown. CT084 is a highly conserved chromosomal gene with homology to phospholipase D. We showed CT084 expression is regulated by Pgp4 and expressed late in the chlamydial developmental cycle. To investigate the function of CT084 in chlamydial lytic exit from infected cells, we made a CT084 null strain (ct084::bla) by using Targetron. The ct084::bla strain grew normally in vitro compared to wild-type strain; however, the strain did not lyse infected cells and produced significantly less and smaller plaques. Collectively, our finding shows Pgp4-regulated CT084-mediated chlamydia lytic exit from infected host cells.

Chlamydia trachomatis plasmid lytic exit phospholipase CT084

SCI

English

Corresponding

National Natural Science Foundation of China[32270203]

Immunology ; Infectious Diseases ; Microbiology

Immunology ; Infectious Diseases ; Microbiology

WOS:001087241800001

OXFORD UNIV PRESS

Web of Science

1.NIAID, Lab Clin Immunol & Microbiol, NIH, Bethesda, MD USA
2.NIAID, Rocky Mt Labs, Hamilton, MT 59840 USA
3.Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB R3T 2N2, Canada
4.Southern Univ Sci & Technol, Sch Publ Hlth & Emergency Management, Room 403,7th Bldg,Chuang Yuan 1088,Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China

Karanovic, Una,Lei, Lei,Martens, Craig A.,et al. TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype[J]. PATHOGENS AND DISEASE,2023,81.

Karanovic, Una.,Lei, Lei.,Martens, Craig A..,Barbian, Kent.,Mcclarty, Grant.,...&Yang, Chunfu.(2023).TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype.PATHOGENS AND DISEASE,81.

Karanovic, Una,et al."TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype".PATHOGENS AND DISEASE 81(2023).