| Title | A comprehensive evaluation of a polymeric zwitterionic hydrophilic monolith for nucleotide separation |
| Author | |
| Corresponding Author | Xu, Dongsheng; Jiang, Zhengjin |
| Publication Years | 2023-10-01
|
| DOI | |
| Source Title | |
| ISSN | 0910-6340
|
| EISSN | 1348-2246
|
| Abstract | Rapid and effective separation of nucleotides (NTs) and their derivatives is crucial for studying their physiological functions. In this work, we comprehensively evaluated the separation ability of a zwitterionic hydrophilic monolith, i.e., poly(N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl)ammonium betaine-co-N,N'-methylenebisacrylamide) (poly(SPP-co-MBA)) for NTs analysis, including its selectivity, chemical stability under extremely basic condition and compatibility with hydrophilic interaction liquid chromatography (HILIC) coupled with mass spectrometry (HILIC-MS). The poly(SPP-co-MBA) monolith exhibited excellent chemical stability, as evidenced by the low relative standard deviation of retention time (0.16-1.05%) after 4000 consecutive injections over one month under strong alkaline elution condition (pH 10). After optimizing the separation conditions, including buffer pH and concentration, organic solvent content and column temperature, four nucleoside triphosphates, five nucleoside diphosphates and five nucleoside monophosphates were baseline separated within 7 min. Additionally, the mixtures containing one nucleoside and its corresponding mono-, di-, and triphosphates were baseline separated within only 3 min, respectively. It is good HILIC-MS compatibility was also confirmed by the satisfactory peak shape and high response of nine NTs. Overall, the proposed poly(SPP-co-MBA) monolith exhibited good mechanical stability and compatibility of HILIC-MS, making it a promising technique for NTs analysis. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Others
|
| Funding Project | National Natural Science Foundation of China["82104115","82173781"]
; Guangdong Basic and Applied Basic Research Foundation[2020A1515110867]
; Science and Technology Innovation Project of Guangdong Drug Administration[2022ZDB04]
; China Postdoctoral Science Foundation[2022M721355]
; Key Field Projects of Guangdong Universities (Intelligent Manufacturing)[2020ZDZX2057]
|
| WOS Research Area | Chemistry
|
| WOS Subject | Chemistry, Analytical
|
| WOS Accession No | WOS:001084097300002
|
| Publisher | |
| ESI Research Field | CHEMISTRY
|
| Data Source | Web of Science
|
| Citation statistics | |
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/582900 |
| Department | The Third People's Hospital of Shenzhen |
| Affiliation | 1.Jinan Univ, Inst Pharmaceut Anal, Coll Pharm,Int Cooperat Lab Tradit Chinese Med Mod, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Peoples R China 2.Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Natl Clin Res Ctr Infect Dis, Dept Clin Lab,Affiliated Hosp 2, Shenzhen 518114, Peoples R China |
| Recommended Citation GB/T 7714 |
Lai, Liang,Zhang, Mengyun,Liu, Chusheng,et al. A comprehensive evaluation of a polymeric zwitterionic hydrophilic monolith for nucleotide separation[J]. ANALYTICAL SCIENCES,2023.
|
| APA |
Lai, Liang,Zhang, Mengyun,Liu, Chusheng,Qu, Jiahuan,Xu, Dongsheng,&Jiang, Zhengjin.(2023).A comprehensive evaluation of a polymeric zwitterionic hydrophilic monolith for nucleotide separation.ANALYTICAL SCIENCES.
|
| MLA |
Lai, Liang,et al."A comprehensive evaluation of a polymeric zwitterionic hydrophilic monolith for nucleotide separation".ANALYTICAL SCIENCES (2023).
|
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