| Title | Site-Specific Modification of Single Domain Antibodies by Enzyme-Immobilized Magnetic Beads |
| Author | |
| Corresponding Author | Zhang, Bo; Wu, Changfeng |
| Publication Years | 2023-10-07
|
| DOI | |
| Source Title | |
| ISSN | 1043-1802
|
| EISSN | 1520-4812
|
| Volume | 34Issue:10 |
| Abstract | Nanobodies as imaging agents and drug conjugates have shown great potential for cancer diagnostics and therapeutics. However, site-specific modification of a nanobody with microbial transglutaminase (mTGase) encounters problems in protein separation and purification. Here, we describe a facile yet reliable strategy of immobilizing mTGase onto magnetic beads for site-specific nanobody modification. The mTGase immobilized on magnetic beads (MB-mTGase) exhibits catalytic activity nearly equivalent to that of the free mTGase, with good reusability and universality. Magnetic separation simplifies the protein purification step and reduces the loss of nanobody bioconjugates more effectively than size exclusion chromatography. Using MB-mTGase, we demonstrate site-specific conjugation of nanobodies with fluorescent dyes and polyethylene glycol molecules, enabling targeted immunofluorescence imaging and improved circulation dynamics and tumor accumulation in vivo. The combined advantages of MB-mTGase method, including high conjugation efficiency, quick purification, less protein loss, and recycling use, are promising for site-specific nanobody functionalization and biomedical applications. |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | National Key R&D Program of China[2020YFA0909000]
; National Natural Science Foundation of China["62235007","22204070"]
; Shenzhen Science and Technology Program["KQTD20170810111314625","SGDX20211123114002003","JCYJ20210324115807021"]
; Shenzhen Bay Laboratory[SZBL2021080601002]
; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]
|
| WOS Research Area | Biochemistry & Molecular Biology
; Chemistry
|
| WOS Subject | Biochemical Research Methods
; Biochemistry & Molecular Biology
; Chemistry, Multidisciplinary
; Chemistry, Organic
|
| WOS Accession No | WOS:001082562300001
|
| Publisher | |
| ESI Research Field | CHEMISTRY
|
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:1
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/582929 |
| Department | Department of Biomedical Engineering |
| Affiliation | 1.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Inst Clin Nucl Med, Sch Med,Dept Nucl Med, Shanghai 200217, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Zhou, Siyu,Fang, Xiaofeng,Lv, Jiahui,et al. Site-Specific Modification of Single Domain Antibodies by Enzyme-Immobilized Magnetic Beads[J]. BIOCONJUGATE CHEMISTRY,2023,34(10).
|
| APA |
Zhou, Siyu.,Fang, Xiaofeng.,Lv, Jiahui.,Yang, Yicheng.,Zeng, Yiqi.,...&Wu, Changfeng.(2023).Site-Specific Modification of Single Domain Antibodies by Enzyme-Immobilized Magnetic Beads.BIOCONJUGATE CHEMISTRY,34(10).
|
| MLA |
Zhou, Siyu,et al."Site-Specific Modification of Single Domain Antibodies by Enzyme-Immobilized Magnetic Beads".BIOCONJUGATE CHEMISTRY 34.10(2023).
|
| Files in This Item: | There are no files associated with this item. | |||||
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