| Title | Genome editing in rice using CRISPR/Cas12i3 |
| Author | |
| Corresponding Author | Zhu, Jian-Kang; Zhang, Jinshan |
| Publication Years | 2023-10-01
|
| DOI | |
| Source Title | |
| ISSN | 1467-7644
|
| EISSN | 1467-7652
|
| Abstract | The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Corresponding
|
| Funding Project | This work was supported by the Key Research and Development Program of Shandong Province (Agricultural Seed Improvement Project, 2022LZGC001).[2022LZGC001]
|
| WOS Research Area | Biotechnology & Applied Microbiology
; Plant Sciences
|
| WOS Subject | Biotechnology & Applied Microbiology
; Plant Sciences
|
| WOS Accession No | WOS:001083191500001
|
| Publisher | |
| ESI Research Field | PLANT & ANIMAL SCIENCE
|
| Data Source | Web of Science
|
| Citation statistics | |
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/582948 |
| Department | Institute of Advanced Biotechnology 生命科学学院 |
| Affiliation | 1.Bellagen Biotechnol Co Ltd, Jinan, Peoples R China 2.Qilu Normal Univ, Sch Life Sci, Jinan, Peoples R China 3.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing, Peoples R China 4.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China 5.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya, Peoples R China 6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China 7.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Peoples R China |
| Corresponding Author Affilication | Institute of Advanced Biotechnology; School of Life Sciences |
| Recommended Citation GB/T 7714 |
Lv, Ping,Su, Fei,Chen, Fangyuan,et al. Genome editing in rice using CRISPR/Cas12i3[J]. PLANT BIOTECHNOLOGY JOURNAL,2023.
|
| APA |
Lv, Ping.,Su, Fei.,Chen, Fangyuan.,Yan, Chunxue.,Xia, Dandan.,...&Zhang, Jinshan.(2023).Genome editing in rice using CRISPR/Cas12i3.PLANT BIOTECHNOLOGY JOURNAL.
|
| MLA |
Lv, Ping,et al."Genome editing in rice using CRISPR/Cas12i3".PLANT BIOTECHNOLOGY JOURNAL (2023).
|
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