In Situ Tyrosinase Monitoring by Wearable Microneedle Patch toward Clinical Melanoma Screening

Huang, Xueqin1,2; Chen, Lingzhi1; Sha, Tianjian1; Lin, Yongjian3; Zeng, Runmin1; Xu, Jun1; Chen, Shanze1; Cai, Huai-Hong3; Zhang, Jianglin1; Zhou, Haibo1; Sun, Ping-Hua1; Jiang, Xingyu4

2023-10-04

10.1021/acsnano.3c05638

ACS NANO   Impact Factor & Quartile

1936-0851

1936-086X

Despite the potential indicating role of tyrosinase (TYR) in cutaneous melanoma, how to capture the real changes of TYR in suspicious skin remains a major challenge. Unlike the traditional human serum test, this study reports a sensing platform that incorporates a wearable microneedle (MN) patch and trimetallic Au@Ag-Pt nanoparticles (NPs) for surface-enhanced Raman scattering (SERS) and colorimetric dual-mode detecting TYR in human skin in situ toward potential melanoma screening. In the presence of TYR, catechol immobilized on MN is preferentially oxidized to benzoquinone, which competitively impedes the interaction of MN and Au@Ag-Pt NPs, triggering the SERS-colorimetric signal reciprocal switch. Using a B16F10 mouse melanoma model, our platform is capable of noninvasively piercing the skin surface and detecting TYR levels before and during anti-PD-1 antibody treatment, which would be highly informative for prognostic judgment and illness monitoring of melanoma. Through in situ sensing for capturing the metabolic changes of TYR in advance, this platform was successfully applied to discriminate the melanoma subjects from skin moles and normal ones (p < 0.001), as well as screen potential melanoma from lactate dehydrogenase (LDH)-negative patients. Melanoma growth and prognosis can still be monitored through recording the continuous change of TYR levels. More importantly, the well-defined flexible and stretchable characteristics of the MN patch allow robustly adhering to the skin without inducing chemical or physical irritation. We believe this platform integrating MN-based in situ sensing, TYR responsiveness, and SERS/colorimetric dual-readout strategy will have high clinical importance in early diagnosis and monitoring of cutaneous melanoma.

tyrosinase surface-enhancedRaman scattering in situ sensing microneedle patch melanoma screening

SCI

English

NI Journal Papers

Others

National Natural Science Foundation of China["82373833","22177039","82304438"] ; National Key Research and Development Program of China[2021YFC2300400] ; Basic and Applied Basic Research Foundation of Guangdong Province["2022A1515110618","2023A1515010789","2022A1515010300"] ; China Postdoctoral Science Foundation[2022M712191]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001078925500001

AMER CHEMICAL SOC

Web of Science

1.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Affiliated Hosp 5,Coll Pharm, Guangzhou 510632, Peoples R China
2.Guangdong Med Univ, Inst Lab Med, Guangdong Prov Key Lab Med Mol Diagnost, Sch Med Technol,Dongguan Affiliated Hosp 1, Dongguan 523000, Peoples R China
3.Jinan Univ, Coll Chem & Mat Sci, Guangzhou 510632, Peoples R China
4.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Shenzhen 518055, Peoples R China

Huang, Xueqin,Chen, Lingzhi,Sha, Tianjian,et al. In Situ Tyrosinase Monitoring by Wearable Microneedle Patch toward Clinical Melanoma Screening[J]. ACS NANO,2023,17(20).

Huang, Xueqin.,Chen, Lingzhi.,Sha, Tianjian.,Lin, Yongjian.,Zeng, Runmin.,...&Jiang, Xingyu.(2023).In Situ Tyrosinase Monitoring by Wearable Microneedle Patch toward Clinical Melanoma Screening.ACS NANO,17(20).

Huang, Xueqin,et al."In Situ Tyrosinase Monitoring by Wearable Microneedle Patch toward Clinical Melanoma Screening".ACS NANO 17.20(2023).