Enhanced bone regeneration via local low-dose delivery of PTH_1-34 in a composite hydrogel

Zhang, Shanyong1; Ding, Lei2; Chen, Gaoyang3; Zhang, Jiayin1; Ge, Wanbao1; Qu, Yuan1

2023-07-03

10.3389/fbioe.2023.1209752

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY   Impact Factor & Quartile

2296-4185

Introducing bone regeneration-promoting factors into scaffold materials to improve the bone induction property is crucial in the fields of bone tissue engineering and regenerative medicine. This study aimed to develop a Sr-HA/PTH1-34-loaded composite hydrogel system with high biocompatibility. Teriparatide (PTH1-34) capable of promoting bone regeneration was selected as the bioactive factor. Strontium-substituted hydroxyapatite (Sr-HA) was introduced into the system to absorb PTH1-34 to promote the bioactivity and delay the release cycle. PTH1-34-loaded Sr-HA was then mixed with the precursor solution of the hydrogel to prepare the composite hydrogel as bone-repairing material with good biocompatibility and high mechanical strength. The experiments showed that Sr-HA absorbed PTH1-34 and achieved the slow and effective release of PTH1-34. In vitro biological experiments showed that the Sr-HA/PTH1-34-loaded hydrogel system had high biocompatibility, allowing the good growth of cells on the surface. The measurement of alkaline phosphatase activity and osteogenesis gene expression demonstrated that this composite system could promote the differentiation of MC3T3-E1 cells into osteoblasts. In addition, the in vivo cranial bone defect repair experiment confirmed that this composite hydrogel could promote the regeneration of new bones. In summary, Sr-HA/PTH1-34 composite hydrogel is a highly promising bone repair material.

PTH bone regeneration hydrogel strontium hydroxyapatite

SCI

English

Others

National Natural Science Foundation of China[82001448] ; Natural Science Foundation of Jilin Province[YDZJ202201ZYTS066] ; Sanming Project of Medicine in Shenzhen[SZSM202111015] ; Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration[ZDSYS20200811143752005]

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Multidisciplinary Sciences

WOS:001031345300001

FRONTIERS MEDIA SA

Web of Science

1.Second Hosp Jilin Univ, Dept Spine Surg, Changchun, Peoples R China
2.Second Hosp Jilin Univ, Dept Rehabil, Changchun, Peoples R China
3.Southern Univ Sci & Technol, Jinan Univ Coll Med 2, Shenzhen Peoples Hosp, Shenzhen Key Lab Musculoskeletal Tissue Reconstruc, Shenzhen, Peoples R China

Zhang, Shanyong,Ding, Lei,Chen, Gaoyang,et al. Enhanced bone regeneration via local low-dose delivery of PTH_1-34 in a composite hydrogel[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2023,11.

Zhang, Shanyong,Ding, Lei,Chen, Gaoyang,Zhang, Jiayin,Ge, Wanbao,&Qu, Yuan.(2023).Enhanced bone regeneration via local low-dose delivery of PTH_1-34 in a composite hydrogel.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,11.

Zhang, Shanyong,et al."Enhanced bone regeneration via local low-dose delivery of PTH_1-34 in a composite hydrogel".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 11(2023).