First identification of multidrug-resistant Acinetobacter bereziniae isolates harboring blaNDM-1 from hospitals in South China

Mo, Xiao-Mei1,3; Pan, Qing2; Seifert, Harald6,7; Xing, Xi-Wen8; Yuan, Jing3; Zhou, Zi-Yuan1,3,9,10; Luo, Xing-Yu5; Liu, Hou-Ming3; Xie, Yong-Li3,4; Yang, Liu-Qing3; Hong, Xiao-Bing1,11,12; Higgins, Paul G.6,7; Wong, Nai-Kei1,3,12

2023

10.1016/j.heliyon.2022.e12365

HELIYON   Impact Factor & Quartile

2405-8440

This study is a first report on the identification of multidrug-resistant (MDR) Acinetobacter bere-ziniae among non-baumannii acinetobacters that had previously escaped automated laboratory detection, and characterize their clinical courses of infection at two tertiary-care hospitals in Shenzhen city, China (2015-2017). Herein, definitive identification by PCR was performed with universal and species-specific primers targeting 16S rDNA and rpoB genes, respectively, followed by Sanger sequencing and blast analysis. Antimicrobial susceptibility of A. bereziniae isolates was assessed accordingly. Three of the five identified A. bereziniae isolates exhibited carbapenem-resistance and were subjected to a multiplex PCR assay to detect drug-resistance genes. Se-quences of the rpoB amplicon were aligned with curated sequences from global databases for phylogenetic analysis on evolutionary relations. Five clinical isolates of A. bereziniae were thereby re-identified, whose infections were primarily nosocomial. Automated identification and sus-ceptibility testing systems (Phoenix-100 and VITEK 2) proved insufficient for discriminating A. bereziniae from other acinetobacters such as Acinetobacter baumannii and Acinetobacter guil-louiae. Among these isolates, three exhibited carbapenem-resistant phenotypes indistinguishable from that of carbapenem-resistant A. baumannii. The carbapenem-resistant A. bereziniae isolates were subsequently confirmed to carry a blaNDM-1 (New Delhi metallo-beta-lactamase-1) gene downstream of ISAba125. Phylogenetic analysis revealed that A. bereziniae isolates evolved slowly but independently in local habitats. A. bereziniae isolates are difficult to distinguish by traditional automated detection systems. PCR-based identification via amplification and sequencing of selected house-keeping genes provides sufficient resolution for discriminating the isolates.

Acinetobacter bereziniae NDM-1 (New Delhi metallo-?-lactamase-1) Healthcare -associated infection Antimicrobial resistance Emerging pathogen

SCI

English

Corresponding

Sanming Project of Medicine in Shenzhen[SZSM2015120005] ; Natural Science Foundation of China[32073002]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000968583700001

CELL PRESS

Web of Science

1.Shantou Univ, Med Coll, Dept Pharmacol, Clin Pharmacol Sect, Shantou, Peoples R China
2.AlphaMol Sci Ltd, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China
4.Shenzhen Univ, Coll Life Sci & Oceanol, Shenzhen Key Lab Microbial Genet Engn, Shenzhen, Peoples R China
5.Zhejiang Chinese Med Univ, Grad Sch, Hangzhou, Peoples R China
6.Univ Cologne, Inst Med Microbiol Immunol & Hyg, Cologne, Germany
7.German Ctr Infect Res DZIF, Partner Site Bonn Cologne, Cologne, Germany
8.Jinan Univ Guangzhou, Coll Life Sci & Technol, Dept Biotechnol, Guangzhou, Peoples R China
9.Chinese Acad Med Sci & Peking Union Med Coll, Canc Hosp, Shenzhen, Peoples R China
10.Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen, Peoples R China
11.Shantou Univ, Med Coll, Affiliated Hosp 2, Dept Pharm, Shantou, Peoples R China
12.Shantou Univ, Med Coll, Guangdong Prov Key Lab Infect Dis & Mol Immunopat, Shantou, Peoples R China

Mo, Xiao-Mei,Pan, Qing,Seifert, Harald,et al. First identification of multidrug-resistant Acinetobacter bereziniae isolates harboring blaNDM-1 from hospitals in South China[J]. HELIYON,2023,9(1).

Mo, Xiao-Mei.,Pan, Qing.,Seifert, Harald.,Xing, Xi-Wen.,Yuan, Jing.,...&Wong, Nai-Kei.(2023).First identification of multidrug-resistant Acinetobacter bereziniae isolates harboring blaNDM-1 from hospitals in South China.HELIYON,9(1).

Mo, Xiao-Mei,et al."First identification of multidrug-resistant Acinetobacter bereziniae isolates harboring blaNDM-1 from hospitals in South China".HELIYON 9.1(2023).