Comprehensive bulk and single-cell transcriptome profiling give useful insights into the characteristics of osteoarthritis associated synovial macrophages

Liao, Shengyou1; Yang, Ming2; Li, Dandan3; Wu, Ye1,4; Sun, Hong5; Lu, Jingxiao5; Liu, Xinying5; Deng, Tingting1; Wang, Yujie1; Xie, Ni5; Tang, Donge3; Nie, Guohui1,6; Fan, Xiaoqin1,5

2023-01-05

10.3389/fimmu.2022.1078414

FRONTIERS IN IMMUNOLOGY   Impact Factor & Quartile

1664-3224

BackgroundOsteoarthritis (OA) is a common chronic joint disease, but the association between molecular and cellular events and the pathogenic process of OA remains unclear. ObjectiveThe study aimed to identify key molecular and cellular events in the processes of immune infiltration of the synovium in OA and to provide potential diagnostic and therapeutic targets. MethodsTo identify the common differential expression genes and function analysis in OA, we compared the expression between normal and OA samples and analyzed the protein-protein interaction (PPI). Additionally, immune infiltration analysis was used to explore the differences in common immune cell types, and Gene Set Variation Analysis (GSVA) analysis was applied to analyze the status of pathways between OA and normal groups. Furthermore, the optimal diagnostic biomarkers for OA were identified by least absolute shrinkage and selection operator (LASSO) models. Finally, the key role of biomarkers in OA synovitis microenvironment was discussed through single cell and Scissor analysis. ResultsA total of 172 DEGs (differentially expressed genes) associated with osteoarticular synovitis were identified, and these genes mainly enriched eight functional categories. In addition, immune infiltration analysis found that four immune cell types, including Macrophage, B cell memory, B cell, and Mast cell were significantly correlated with OA, and LASSO analysis showed that Macrophage were the best diagnostic biomarkers of immune infiltration in OA. Furthermore, using scRNA-seq dataset, we also analyzed the cell communication patterns of Macrophage in the OA synovial inflammatory microenvironment and found that CCL, MIF, and TNF signaling pathways were the mainly cellular communication pathways. Finally, Scissor analysis identified a population of M2-like Macrophages with high expression of CD163 and LYVE1, which has strong anti-inflammatory ability and showed that the TNF gene may play an important role in the synovial microenvironment of OA. ConclusionOverall, Macrophage is the best diagnostic marker of immune infiltration in osteoarticular synovitis, and it can communicate with other cells mainly through CCL, TNF, and MIF signaling pathways in microenvironment. In addition, TNF gene may play an important role in the development of synovitis.

osteoarthritis synovium immune infiltration macrophage Scissor analysis

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English

Others

Immunology

Immunology

WOS:000913457800001

FRONTIERS MEDIA SA

Web of Science

1.Shenzhen Univ, Shenzhen Key Lab Nanozymes & Translat Canc Res, Dept Otolaryngol, Shenzhen Inst Translat Med,Affiliated Hosp 1,Shenz, Shenzhen, Guangdong, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp 1, Dept Otolaryngol, Affiliated Hosp, Shenzhen, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Clin Med Coll 2, Affiliated Hosp 1,Shenzhen Peoples Hosp,Jinan Univ, Shenzhen, Peoples R China
4.Guangzhou Med Univ, Sch Basic Med Sci, Dept Otolaryngol, Guangzhou, Guangdong, Peoples R China
5.Shenzhen Univ, Biobank Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
6.Peking Univ Shenzhen Grad Sch, State Key Lab Chem Oncogen, Guangdong Prov Key Lab Chem Genom, Shenzhen, Guangdong, Peoples R China

Liao, Shengyou,Yang, Ming,Li, Dandan,et al. Comprehensive bulk and single-cell transcriptome profiling give useful insights into the characteristics of osteoarthritis associated synovial macrophages[J]. FRONTIERS IN IMMUNOLOGY,2023,13.

Liao, Shengyou.,Yang, Ming.,Li, Dandan.,Wu, Ye.,Sun, Hong.,...&Fan, Xiaoqin.(2023).Comprehensive bulk and single-cell transcriptome profiling give useful insights into the characteristics of osteoarthritis associated synovial macrophages.FRONTIERS IN IMMUNOLOGY,13.

Liao, Shengyou,et al."Comprehensive bulk and single-cell transcriptome profiling give useful insights into the characteristics of osteoarthritis associated synovial macrophages".FRONTIERS IN IMMUNOLOGY 13(2023).