SIRT5 reduces the inflammatory response and barrier dysfunction in IL-17A-induced epidermal keratinocytes

Wang, Chunhui1; He, Danhua2; Shi, Cuiping3,4,5

2023

10.15586/aei.v51i1.675

ALLERGOLOGIA ET IMMUNOPATHOLOGIA   Impact Factor & Quartile

0301-0546

1578-1267

Psoriasis is a chronic multisystemic inflammatory disease with inflammatory cell infiltration, hyperproliferation of keratinocytes in skin lesions, and epidermal barrier dysfunction. Normal human epidermal keratinocytes (NHEKs) were stimulated with interleukin 17A (IL-17A). The expression levels of sirtuin-5 (SIRT5) were analyzed by RT-qPCR and western blot assay. The proliferation levels of NHEKs were assessed by EdU staining. The expression of ELOVL1 and ELOVL4 was analyzed by RT-Qpcr, and the expression levels of filaggrin, loricrin, and aquaporin-3 were analyzed by RT-qPCR and western blot. Extracellular signal-regulated kinase 1/2 (ERK1/2) activator t-butylhydroquinone was used to activate ERK1/2. Here, we show that SIRT5 overexpression reduces cell viability and cell proliferation, and improves barrier dysfunction in IL-17A-treated human epidermal keratinocytes, this effect of which is significantly blunted by the ERK1/2 activator. In epidermal keratinocytes, SIRT5 decreases cell proliferation and inflammation and improves barrier dysfunction via ERK/STAT3. This study reveals the role of SIRT5 in the pathogenesis of psoriasis, epidermal hyperplasia, keratinocyte-mediated inflammatory responses, and barrier dysfunction, the role of which is mediated by ERK/STAT3. (c) 2023 Codon Publications. Published by Codon Publications.

Dysfunction Epidermal Barrier ERK STAT3 Inflammation Proliferation Psoriasis SIRT5

SCI

English

Corresponding

Allergy ; Immunology

Allergy ; Immunology

WOS:000910922200005

CODON PUBLICATIONS

IMMUNOLOGY

Web of Science

1.Southern Med Univ, Pingshan Dist Peoples Hosp Shenzhen, Dept Dermatol, Pingshan Gen Hosp, Shenzhen, Guangdong, Peoples R China
2.Jinan Univ, Shenzhen Baoan Womens & Childrens Hosp, Dept Dermatol, Shenzhen, Guangdong, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Dept Dermatol, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
5.Shenzhen Peoples Hosp, Dermatol Dept, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China

Wang, Chunhui,He, Danhua,Shi, Cuiping. SIRT5 reduces the inflammatory response and barrier dysfunction in IL-17A-induced epidermal keratinocytes[J]. ALLERGOLOGIA ET IMMUNOPATHOLOGIA,2023,51(1).

Wang, Chunhui,He, Danhua,&Shi, Cuiping.(2023).SIRT5 reduces the inflammatory response and barrier dysfunction in IL-17A-induced epidermal keratinocytes.ALLERGOLOGIA ET IMMUNOPATHOLOGIA,51(1).

Wang, Chunhui,et al."SIRT5 reduces the inflammatory response and barrier dysfunction in IL-17A-induced epidermal keratinocytes".ALLERGOLOGIA ET IMMUNOPATHOLOGIA 51.1(2023).